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ABSTRACT:
PROVIDER: PRJNA496308 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We monitored by RNAseq the transcriptomic response of roots and leaves of Triticum aestivum cv chinese Spring during a long term interaction with Funneliformis mossae (2 months) with or without a pathogen infection by infiltration of Xanthomonas translucens CFBP 2054. The control condition of roots and leaves wheat without mycorhizal fungi is in E-MTAB-5891 (material produced simultaneously and treated at the same time).
2018-06-01 | E-MTAB-5898 | biostudies-arrayexpress
Project description:This experiment was designed to study the interactions between Medicago truncatula and the charcoal rot pathogen Macrophomina phaeolina. Two-week-old plants grown in Magenta boxes supplied with 1/2 MS salt and 1% sucrose were inoculated with M. phaseolina covered wheat seeds, and roots were harvested at 24, 36 and 48 hours after inoculation. Control plants were mock inoculated with a sterile wheat seed, and roots were harvest 24 hours later. Pooled RNAs were used in the array experiment using Affymetrix GeneChip(r) Medicago Genome Array.
2012-05-15 | E-MEXP-3580 | biostudies-arrayexpress
Project description:This study aimed at decrypting the transcriptomic response of 2 months-old grown tender wheat (cv Chinese Spring) to a the Xanthomonas translucens pathogen infection by infiltration. The response was monitored by RNAseq 24h post leaf clipping. Triticum aestivum cv. Chinese Spring plants were maintained in a growth chamber with cycles of 12 h of light at 21C and 50% relative humidity (RH) and 12 h of dark at 21C and 50% RH. Leaves of 49 days-old plants were infiltrated with a bacterial suspension in water with an optical density at 600 nm (OD600) of 0.5 using a needleless syringe. Plants inoculated with water were used as controls. For transcriptomic and proteomic analyses, leaves and root tissues were harvested 1 day post-inoculation (dpi), when symptoms were not visible yet. Three biological replicates per treatment were performed, and each with pooled leaves from two independent plants per replicate. The files per conditions and replicates are:Sample 1 Root tissue with 3 replicates: CONTROL * control condition for roots (wheat without pathogen infection): 3 replicates: 1.1R,1.2R, 1.3R * control condition for leaves (wheat without pathogen infection): 3 replicates1.1L,1.2L, 1.3L * Wheat Roots infected by Xanthomonas translucens: 3 replicates: 5.1R, 5.2R, 5.3R * Wheat Leaves infected by Xanthomonas translucens: 3 replicates: 5.1L, 5.2L, 5.3L
2017-09-01 | E-MTAB-5891 | biostudies-arrayexpress
Project description:Plant growth promoting bacteria (PGPB) might be an alternative to increase nitrogenous use efficiency (NUE) in important crops such wheat. Azospirillum brasilense is one of the most promising PGPB and wheat roots colonized by Azospirillum brasilense is a good model to investigate the molecular basis of plant-PGPB interaction including improvement in plant-NUE promoted by PGPB. An RNA-seq transcriptional analysis of Triticum aestivum roots was carried out in two independent samples (biological replicates) of each treatment (PGPB-colonized or non-inoculated), yielding a total of 4 sequencing libraries, which were designated CWR1 and CWR2 libraries (colonized roots) and N-IWR1 and N-IWR2 (non-inoculated roots).
2014-04-10 | E-MTAB-2301 | biostudies-arrayexpress
Project description:Mycosphaerella graminicola is the causal agent of Mycosphaerella graminicola infection (STB) disease of wheat. Wheat genotypes vary in their response to this disease. Cultivar (cv.) Longbow is susceptible and cv. Flame is resistant to STB disease, with cv. Flame possessing the STB resistance locus Stb6 that confers resistance to pathogen strain IPO323. Gene expression profiling (conducted using Affymetrix wheat gene chip) identified transcripts that accumulate in leaves of both these wheat cultivars as an early response to M. graminicola strain IPO323 (at 24h post-treatment). At this initial time point, microscopic analysis verified that fungal spores had germinated, but not penetrated the leaves of both genotypes. Results showed that basal defence genes were activated in both the compatible and incompatible interactions. A subset of genes were identified that were more pathogen-responsive in the cv. Flame v. IPO323 incompatible interaction as compared to the cv. Longbow v. IPO323 compatible interaction, including defence genes such as peroxidases, beta-1,3-glucanase, annexin, chitinases, brassinosteroid-associated kinase 1 and a jasmonate-inducible protein.
2014-06-04 | E-MEXP-3584 | biostudies-arrayexpress
Project description:Abstract Background: Fusarium crown rot is major disease in wheat. However, the wheat defense mechanisms against this disease remain poorly understood. Results: Using tandem mass tag (TMT) quantitative proteomics, we evaluated a disease-susceptible (UC1110) and a disease-tolerant (PI610750) wheat cultivar inoculated with Fusarium pseudograminearum WZ-8A. The morphological and physiological results showed that the average root diameter and malondialdehyde content in the roots of PI610750 decreased 3 days post-inoculation (dpi), while the average number of root tips increased. Root vigor was significantly increased in both cultivars, indicating that the morphological, physiological, and biochemical responses of the roots to disease differed between the two cultivars. TMT analysis showed that 366 differentially expressed proteins (DEPs) were identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment in the two comparison groups, UC1110_3dpi/UC1110_0dpi (163) and PI610750_3dpi/PI610750_0dpi (203). It may be concluded that phenylpropanoid biosynthesis (8), secondary metabolite biosynthesis (12), linolenic acid metabolites (5), glutathione metabolism (8), plant hormone signal transduction (3), MAPK signaling pathway-plant (4), and photosynthesis (12) contributed to the defense mechanisms in wheat. Protein-protein interaction network analysis showed that the DEPs interacted in both sugar metabolism and photosynthesis pathways. Sixteen genes were validated by real-time quantitative polymerase chain reaction and were found to be consistent with the proteomics data. Conclusion: The results provided insight into the molecular mechanisms of the interaction between wheat and F. pseudograminearum.
2021-01-04 | PXD023314 | Pride
Project description:To gain comprehensive information on the molecular and physiological bases during particular stages of the interaction between wheat and S. tritici, we explored the complexity of transcriptional reprogramming of both host and pathogen at three time points (day 4, 10 and 13) by RNA-Seq analysis. The time points were selected based on the bioassay of marker genes/proteins and H2O2 accumulation and represented the initial symptomless phase, the intermediate transition stage and the necrotrophic phase, respectively.
2013-05-30 | E-MTAB-4470 | biostudies-arrayexpress
Project description:The influence of arbuscular mycorrhizal (AM) colonization on the expression of genes in the roots of wheat (Triticum aestivum L.) at the transcriptome level is largely unknown. A pot experiment was established to study the responses of the transcriptome profile in the roots of wheat to colonization by the AM fungus Rhizophagus irregularis using high through-put sequencing methods. The results indicated that the expression of 11,746 genes was regulated by AM colonization, and 64.7% of them were up-regulated genes. 1106 genes were only expressed in roots colonized by AMfungi, and 108 genes were only expressed in non- mycorrhizal roots. The differentially expressed genes (DEGs) were primarily distributed on the 2B, 3B, 2A, 2D, and 5B chromosomes of wheat. The DEGs (including both up- and down- regulated) mainly located on membranes, and functioned in nucleotide binding and transferase activity during cellular protein modification and biosynthetic processes. The data revealed that AMcolonization up-regulated genes involved in the phenylpropanoid biosynthesis pathway and transcription factors which play vital roles in protecting plants from biotic or abiotic stresses. A number of key genes involved in molecular signal biosynthesis and recognition, epidermal cell colonization and arbuscule formation, carbon and nutrients exchange during AM symbiosis were found. All the ammonium transporter (AMT), iron-phytosiderophore transporter, boron, zinc, and magnesium transporter genes found in our study were up-regulated DEGs. One new AM-specific induced AMT and three new AM-specific induced nitrate transporter (NRT) genes were found in the roots of wheat colonized by AMfungi, even though a negative growth response of wheat to AM colonization occurred. The present study provided new information which is important for under- standing the mechanisms behind the development and function of the symbiosis between wheat and AM fungi.
2019-03-30 | GSE129059 | GEO
Project description:Contrary to the relative wealth of information regarding pathogen defense responses in aboveground plant parts, little is known about the mechanistic basis and regulation of plant immunity in root tissues. Aiming to further our fundamental understanding of root immune responses, we have investigated the interaction between rice and one of its major root pathogens, the oomycete Pythium graminicola. The specificic objectives of this study were twofold: i) to disentangle the molecular and genetic basis of the rice-Pythium interaction by comparing the transcriptome of rice roots at different times after inoculation with a highly virulent Pythium strains, and ii) to offer fundamental insights into the genetic architecture and regulation of rice disease resistance pathways operative in root tissue and to identify the molecular players controlling the possible nodes of convergence between these resistance conduits Comparison between P. graminicola- and mock-infected rice roots. Two treatments (infected and non-infected) x three timepoints (1, 2 and 4 days post inoculation) x three biological replicates
2011-10-03 | E-GEOD-32582 | biostudies-arrayexpress
Project description:Barley contains a much higher content of bioactive substances than wheat. In order to investigate the effect of genome interaction between barley and wheat on phytosterol content, we used a series of barley chromosome addition lines of common wheat. The wheat 38k-microarray was utilized for screening of genes with expression levels specifically increased by an additive effect or synergistic action between wheat and barley chromosomes. We determined the overall expression pattern of genes related to phytosterol biosynthesis in wheat and in each addition line. Together with determining the phytosterol levels of wheat, barley and each addition line, we assess the critical genes in the phytosterol pathway that can be expressed to promote phytosterol levels.
2011-12-08 | GSE28023 | GEO