Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

ABSTRACT: Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

PROVIDER: PRJNA497250 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells [Mouse SMART-seq2]

Project description:IL-10 is a prototypical anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4+ T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3Neg CD4+ T cells that displays regulatory activity unlike other IL-10-producing CD4+ T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4+ T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3Neg CD4+ T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease (IBD), demonstrate a deficiency in this specific regulatory T-cell subpopulation.

2018-11-07 | GSE121386 | GEO

Smart-seq2 single-cell RNA sequencing of mouse liver non-parenchymal cells

Project description:Smart-seq2 scRNA-seq of liver non-parenchymal cells from 3 high-fat diet and 3 control mice.

2023-04-29 | GSE230440 | GEO

In situ transcriptome characteristics are lost following culture adaptation of adult cardiac stem cells (Smart-Seq2 scRNA-seq)

Project description:Single-cell RNA-seq (Smart-Seq2) to profile of cardiac progenitor cells

2018-09-12 | GSE114279 | GEO

Single-cell RNAseq (SMART-seq2) of wild-type (TLAB) and MZoep (tz57) zebrafish embryos at 50% epiboly stage

Project description:SMART-seq2 was performed on single cells isolated from visually staged zebrafish embryos.

2018-04-27 | GSE106466 | GEO

Smart-seq2 analysis of P17 FACS sorted retinal cells from the Kcng4-cre;stop-YFP X Thy1-stop-YFP Line#1 mice

Project description:Four Kcng4-cre;stop-YFP mouse retinas from two mice were dissected, dissociated and FACS sorted, and single cell RNA-seq libraries were generated for 384 single cells using Smart-seq2. Aligned bam files are generated for 383 samples as one failed to align. Four mouse retinas (labeled 1la, 1Ra, and 2la, 2Ra respective from the two mice) were used, and 96 single cells from each were processed using Smart-seq2. Total 384 cells Smart-seq2 analysis of P17 FACS sorted retinal cells from the Kcng4-cre;stop-YFP mice (Kcng4tm1.1(cre)Jrs mice [Duan et al., Cell 158, 793-807, 2015] crossed to the cre-dependent reporter Thy1-stop-YFP Line#1 [Buffelli et al., Nature 424, 430-434, 2003])

2016-08-25 | E-GEOD-81903 | biostudies-arrayexpress

[Smart-seq2] scRNA-seq of mouse Plasmodium-specific CD4+ T cells

Project description:[Smart-seq2] scRNA-seq of mouse Plasmodium-specific CD4+ T cells

| PRJEB40000 | ENA

Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on C1 System

Project description:In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. We perform scRNA-seq on a homogenous population of mouse embryonic stem cells along with two kinds of control spike-in molecules to assess sensitivity and accuracy of these specific methods. In this dataset, we perform Smart-Seq2 method on Fluidigm C1 system and generate single-cell libraries using Nextera XT kit

2017-03-29 | E-MTAB-5485 | biostudies-arrayexpress

Molecular and functional heterogeneity of IL-10-producing CD4+ T cells.

Project description: Not available

| S-EPMC6303294 | biostudies-literature

Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on C1 system (Replicate run)

Project description:In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. We perform scRNA-seq on a homogenous population of mouse embryonic stem cells along with two kinds of control spike-in molecules to assess sensitivity and accuracy of these specific methods. In this dataset, we perform a replicate of Smart-Seq2 method on Fluidigm C1 system and generate single-cell libraries using Nextera XT kit

2017-03-29 | E-MTAB-5484 | biostudies-arrayexpress

Smart-seq2 analysis of P17 FACS sorted retinal cells from the Tg(Chx10-EGFP/cre,-ALPP)2Clc or Vsx2-GFP transgenic line

Project description:Three Vsx2-GFP mouse retinas were dissected, dissociated and FACS sorted, and single cell RNA-seq libraries were generated for 288 single cells and 3 bulk libraries using Smart-seq2 (~10,000 cells each)

2016-08-25 | GSE80232 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data