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Gene expression profiling U87 cells knocking down Glud1 under low glucose treatment

ABSTRACT: Gene expression profiling U87 cells knocking down Glud1 under low glucose treatment

PROVIDER: PRJNA497832 | ENA |

REPOSITORIES: ENA

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Gene expression profiling U87 cells knocking down Glud1 under low glucose treatment

Project description:U87 cells (shGlud1 and shNT) were treated with low glucose for 6 hours, then mRNA were extracted for high-throughput sequencing

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RNA-sequencing analysis of VEGFR2 knocking down in retinal pigment epithelial (RPE) cells upon glucose depletion.

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Expression data from C2C12 cells after knocking-down lncRNA-MEG3

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RNA-seq Analysis of mRNA profiles in Purβ-overexpressing and Purβ-knocking down hepatocytes

Project description:Purpose: The goal of this study is to investigate how Purβ regulates hepatic glucose production in primary hepatocytes. Methods: mRNA profiles of Purβ-overexpressing, and Purβ-knocking down hepatocytes were generated by deep sequencing using an Illumina NovaSeq 6000 platform. Paired-end clean reads were aligned to the mouse reference genome(Ensemble_GRCm38.96) with TopHat (version 2.0.12), and the aligned reads were used to quantify mRNA expression by using HTSeq-count (version 0.6.1). Conclusion: Our study represents the first detailed analysis of mRNA profiles in Purβ-overexpressing, and Purβ-knocking down hepatocytes, generated by RNA-seq technology. Our results identified Adcy6 was a target of Purβ. RNA-seq analysis showed that Purβ overexpression increased Adcy6 expression, whereas Purβ knockdown decreased Adcy6 expression.

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