Project description:F4/80+ macrophages treated with TGFb2 are potently tolerogenic. Our understanding of the molecular mechanisms mediating the development of these tolerogenic properties is incomplete. We used microarray analysis to identify molecules that are involved in the tolerogenic mechanisms in murine TGFb-treated macrophages.
Project description:F4/80+ macrophages treated with TGFb2 are potently tolerogenic. Our understanding of the molecular mechanisms mediating the development of these tolerogenic properties is incomplete. We used microarray analysis to identify molecules that are involved in the tolerogenic mechanisms in murine TGFb-treated macrophages. Thioglycolate-elicited peritoneal exudate cells (F4/80+ macrophages) were treated with either PBS or TGFb2 overnight then total RNA extracted and subjected to microarray analysis.
Project description:Macrophages are at the front line of the innate immune system and differentiate from monocytes after infiltrating the infected tissue. Metabolic pathways are central to macrophage biology. We studied transcriptional differences between Rapamycin treated in vitro activated macrophages and untreated peripheral blood derived in vitro actiavted macrophages. We used microarrays to detail the global programme of gene expression in Rapamycin treated adult blood derived M(IL-10) and M(IFN-γ) macrophages and untreated adult blood derived M(IL-10) and M(IFN-γ)macropahges
Project description:Analysis of gene expression in macrophages infected with influenza A virus or Mock and treated with the VX-787 to investigate the effects VX-787 have on transcriptional response in human macrophages.