Project description:An in-depth characterization of an observed interspecies interaction between two co-isolated bacteria, Xanthomonas retroflexus and Paenibacillus amylolyticus. Using microsensor measurements for mapping the chemical environment, we show how X. retroflexus promoted an alkalization of its local environment through degradation of amino acids and release of ammonia. When the two species were grown in proximity, the modified local environment induced a morphological change and increased growth of P. amylolyticus followed by sporulation. 2D spatial metabolomics enabled visualization and mapping of the degradation of oligopeptide structures by X. retroflexus and morphological changes of P. amylolyticus through e.g. the release of membrane-associated metabolites. Proteome analysis and microscopy were used to validate the shift from vegetative growth towards sporulation. In summary, we demonstrate how environmental profiling by combined application of microsensor, microscopy, metabolomics and proteomics approaches can reveal growth and sporulation promoting effects resulting from interspecies interactions.

2022-05-20 | PXD030998 | Pride

Paenibacillus amylolyticus strain:NBRC 15957

Project description:Paenibacillus amylolyticus NBRC 15957 genome sequencing project

| PRJDB3958 | ENA

Draft genome of Paenibacillus amylolyticus strain Sp949

Project description:Draft genome of Paenibacillus amylolyticus strain Sp949

| PRJEB15262 | ENA

2022-05-20 | PXD031026 | Pride

Paenibacillus amylolyticus strain:Heshi-A3

Project description:Draft genome sequencing of Paenibacillus amylolyticus Heshi-A3, isolated from a traditional fermented seafood in Japan.

| PRJDB3831 | ENA

Halorussus amylolyticus strain:YC93