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PolyA RNA Sequencing Analysis of HTR-8/SVneo cells after lnc-SLC4A1-1 overexpression

ABSTRACT: polyA RNA Sequencing Analysis of HTR-8/SVneo cells after lnc-SLC4A1-1 overexpression

PROVIDER: PRJNA499120 | ENA |

REPOSITORIES: ENA

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polyA RNA Sequencing Analysis of HTR-8/SVneo cells after lnc-SLC4A1-1 overexpression

Project description:To further evaluate the functional role of lnc- SLC4A1-1, we overexpressed lnc-SLC4A1-1 in HTR-8/SVneo cells and isolated RNA for RNA-seq. RNA-seq was done using TruSeq Stranded mRNA Library Preparation. Briefly, intact RNA was fragmented, end repaired, adapter ligation and PCR amplified following illumina protocol. Libraries were sequencing by illumins Hiseq 3000. After quality control, sequence data were processed with STAR to generate read alignments with hg19. Raw read counts for annotated genes were obtained with featureCounts with default settings, normalized and analyzed using DEGseq.

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Effect of IL-11 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

Project description:IL-11 increases the invasiveness of JEG-3 cells while, reduces the invasiveness of HTR-8/SVneo cells. This study examines the effect of IL-11 on gene expression in trophobalstic cell models viz. JEG-3 and HTR-8/SVneo cells to resolve the controversies associated with the IL-11 mediated regulation of the invasiveness of these two cell lines. JEG-3 and HTR-8/Svneo cells were stimulated with IL-11 (200 ng/ml) for 24 h in pain medium keeping un-treated cells as control. After 24 h of stimulation, total RNA was isolated from these cells and used for the microarray experiments. These experiments were performed once.

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Project description:Both leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) increase the invasiveness of JEG-3 and HTR-8/SVneo cells. This study examines the effect of LIF and IL-6 on gene expression in trophoblastic cell models viz. JEG-3 and HTR-8/SVneo cells to decipher the molecular basis of the increase in invasiveness. JEG-3 and HTR-8/Svneo cells were stimulated with LIF (50 ng/ml) and IL-6 (100 ng/ml) for 24 h in plain medium keeping untreated cells as a control. After 24 h of stimulation, total RNA was isolated from these cells and used for microarray experiments. These experiments were performed once.

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