Ontology highlight
ABSTRACT:
PROVIDER: PRJNA503532 | ENA |
REPOSITORIES: ENA
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Project description:The purpose of this study was the identification of RNAs contained in the urinary exosome (UExo) from dogs and cats. The quality of total RNA in isolated urinary exosome (UExo)-derived total RNAs obtained from the column-based method (urine 1 mL) was checked by using a Bioanalyzer, and samples from normal renal function (NR) group and kidney disease (KD) group were pooled as one sample for each group. We collected NR dogs (n = 37), KD dogs (n = 47), NR cats (n=43), and KD cats (n = 45). For the next generation sequencing, libraries were prepared according to the manufacturer’s protocols and sequenced using 50-base reads acquired by using a HiSeq 2000 platform. The December 2011 (GRCm38/mm10) mouse (Mus musculus) genome data were used as reference. As a result, we could identify the miRNA from these samples.
2018-12-10 | GSE121156 | GEO
Project description:While insulin replacement therapy restores the health and prevents the onset of diabetic complications (DC) for many decades, some T1D patients have elevated hemoglobin A1c values suggesting poor glycemic control, a risk factor of DC. We surveyed the stool microbiome and urinary proteome of a cohort of 220 adolescents and children, half of which had lived with T1D for an average of 7 years and half of which were healthy siblings. Phylogenetic analysis of the 16S rRNA gene did not reveal significant differences in gut microbial alpha-diversity comparing the two cohorts. The urinary proteome of T1D patients revealed increased abundances of several lysosomal proteins that correlated with elevated HbA1c values. In silico protein network analysis linked such proteins to extracellular matrix components and the glycoprotein LRG1. LRG1 is a prominent inflammation and neovascularization biomarker. We hypothesize that these changes implicate aberrant glycation of macromolecules that alter lysosomal function and metabolism in renal tubular epithelial cells, cells that line part of the upper urinary tract.
2019-03-28 | PXD012413 | Pride
Project description:This study is to identify urinary exosome microRNAs (miRNAs) that are unique to premature ovarian insufficiency (POI) with and without Turner syndrome and to use them as diagnostic markers for POI patients. We examined the miRNAexpression profile in urine exosomes from POI patients with and without Turner syndrome.
2022-09-28 | GSE205700 | GEO
Project description:Urinary bladder wound healing is today pooorly chracterized. MicroRNAs are small non-coding RNA molecules with regulatory functions. In this study we aimed at identifying microRNAs expressed during bladder wound healing. We performed Affymetrix microRNA profiling of the rodent urinary bladder during healing of a surgically created wound.
2021-12-01 | GSE176515 | GEO
Project description:Abstract Introduction: Type 1 diabetes (T1D) is a serious autoimmune disease with high morbidity and mortality. Early diagnosis and treatment remain unsatisfactory. Circulating exosomes containing T1D biomarkers have gained interest, but progress is limited. Objectives: This study investigates molecular dynamics of plasma exosomes in pediatric T1D patients and reveals potential mechanisms associated with T1D development. Methods: Liquid chromatography-tandem mass spectrometry with TMT6 labeling quantified exosomal protein expression profiles in 12 healthy and 24 pediatric T1D patients, stratified by age (≤6 years and >6 years) and glycated hemoglobin (HbA1c) levels (>7% or ≤7%). Integrative bioinformatics analyses were conducted, and Western Blot (WB) validated findings. Results: We identified 1035 proteins, revealing 548 and 589 differentially expressed proteins (fold change >1.3) in T1D patients aged ≤6 and >6 years, respectively. When HbA1c was below 7% (received insulin therapy for at least three months), most of the altered protein levels in both age groups resembled healthy controls. Bioinformatics analysis indicated exosome components related to immune functions, hemostasis, cellular stress response, and extracellular matrix organization. WB confirmed alterations in RAB40A, COL6A5, SEMA6D, and TTR proteins. Conclusion: Our study characterizes plasma exosome protein dynamics in pediatric T1D patients across age stages, providing valuable insights into molecular mechanisms and identifying potential therapeutic targets for T1D management.
2023-06-20 | PXD043146 | iProX