Project description:Cultivated soybean has domesticated in China for a long history, and there are several significant phenotypic differences between wild and cultivated soybeans. Seed of cultivar is generally larger than wild soybean, therefore here we comprehensively analyzed transcriptomes of thirteen soybean accessions seeds including seven wild soybeans and six landraces through applying strand-specific RNA sequencing. Differential expressed genes related seed weight were identified, some of them were known to be associated with seed development in Arabidopsis. Noncoding RNAs are known to play important roles in plant development, and we profiled the expression pattern of long noncoding RNA (lncRNA) in cultivated and wild soybean seeds. We have identified 1,251 long intergenic noncoding RNA, 243 intronic RNA and 81 antisense lncRNA, transcriptional levels of a number of lncRNAs were significantly different between cultivated and wild soybeans, suggesting that lncRNA may be involved in soybean seed development.
Project description:Purpose: The goals of this study are to identify what gene GmRAV1 was related to. RNA-Sequencing experiments were designed between GmRAV1-ox soybeans and DN50 soybeans. Methods: Leaves mRNA of 30-day-old DN50 and GmRAV1-ox soybeans were used by RNA sequencing using Illumina HiSeqTM2000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with TopHat followed by Cufflinks. Genome_build: Glycine max Wm82.a2.v1 Results: RNA-seq data confirmed that there are 1467 genes with significant differences. Some genes expression of cytokinin and auxin signaling pathways is dramatically changed, such as GmARR9, GmAUX22, GmPIN1, GmCycD3 and GmPDV2. Conclusions: GmRAV1 are related to cytokinin and auxin signaling pathways.