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Transcriptome differences between brown adipose tissues of wildtype and UCP1-KO mice

ABSTRACT: Transcriptome differences between brown adipose tissues of wildtype and UCP1-KO mice

PROVIDER: PRJNA503812 | ENA |

REPOSITORIES: ENA

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Transcriptome differences between brown adipose tissues of wildtype and UCP1-KO mice

Project description:We analyzed coding transcript abundance in murine, interscapular brown adipose tissue from wildtype and UCP1-KO mice.

2018-11-06 | GSE122133 | GEO

Transcriptome differences between primary brown adipocytes of wildtype and UCP1-KO mice

Project description:We analyzed coding transcript abundance in fully differentiated, primary brown adipocytes from murine, interscapular brown adipose tissue of wildtype and UCP1-KO mice.

2018-09-13 | GSE119873 | GEO

Transcriptome differences between primary brown adipocytes of wildtype and UCP1-KO mice

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Project description:Mice expressing Cas9 were administered AAV8-UCP1 sgRNA to the brown adipose tissue for inducible knockout. Mice were housed at 22ºC for two weeks and then were sacrificed. Brown adipose tissue (BAT) was harvested and RNAseq was performed to determine transcriptional changes stemming from inducible UCP1 knockout.

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Transcriptome differences between white and brown adipose tissue from wildtype and uncoupling protein 1 knockout mice exposed to different diets and ambient temperatures

Project description:We analyzed transcript abundance in interscapular brown and inguinal white adipose tissue of wildtype and UCP1-KO mice either adpated to 20°C or 30°C and fed a high fat or control diet.

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RNA-Sequencing of wild type and UCP1-null beige adipose tissue

Project description:Since brown adipose tissue (BAT) dissipates energy through UCP1, BAT has garnered attention as a therapeutic intervention for obesity and metabolic diseases including type 2 diabetes. As we better understand the roles of classical brown and beige adipocytes, increased beige fat mass in response to a variety of external/internal cues is associated with significant improvements in glucose and lipid homeostasis that may not be entirely mediated by UCP1. We aim to analyze transcriptome of wild type and UCP1-null beige adipocyte to identify the UCP1-independent function.

2017-10-28 | E-MTAB-4085 | biostudies-arrayexpress

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Project description:Abstract Brown and brite adipocytes are the key cells performing uncoupling protein 1 (UCP1) dependent non-shivering thermogenesis (NST) induced by cold exposure. Several lipid species are associated to NST in brown and white adipose tissue. Studies investigating the association of the lipid profile with NST rely on the analysis of whole organ homogenates or on the differentiation of pre-adipocytes in vitro. These approaches have so far not addressed the heterogeneity of white adipose tissue. Aim of this study was to characterize the lipid composition of white adipose tissue on a region-specific level in an in vivo context. We applied MALDI mass spectrometry imaging (MALDI-MSI) in combination with immunohistochemistry and high-resolution mass spectrometry on sections of inguinal white adipose tissue of 129S6/SvEvTac and C57BL6/N-UCP1 knockout and wildtype mice acclimatized to cold to identify lipids specific to areas of UCP1 expression. Based on the analysis of cold exposed 129S6/SvEvTac mice we identified cardiolipins (CL) and diacylglycerols (DG) species to be specific for areas expressing UCP1 and triacylglycerols (TG) to be the main lipid class characteristic for UCP1 negative regions within inguinal white adipose tissue. Investigation of C57BL6/N-UCP1 knockout and wildtype mice housed at either room temperature or acclimatized to cold, demonstrated that CL content in white adipose tissue is increased upon cold stimulation, independent of UCP1. We introduce a MALDI-MSI based approach to identify lipids associated to thermogenic adipocytes in adipose tissues demonstrating a clear regional cold dependent upregulation of CL independent of UCP1.

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Immortalized clonal brown, beige and white adipose cell lines

2012-07-24 | GSE39562 | GEO

Immortalized clonal brown, beige and white adipose cell lines

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