ABSTRACT: Isolation of the first New Delhi metallo-ß-lactamase-1 (NDM-1) producing, colistin resistant, Klebsiella pneumoniae sequence type ST15, from a digestive carrier in Albania, May 2018
Project description:Genome sequencing of New Delhi metallo-beta-lactamase (NDM)-producing K. pneumoniae isolates from a large regional outbreak in Italy, 2018- 2019
Project description:The study aims at sequencing a multi-drug resistant, New Delhi Metallo-β-Lactamase-1 (NDM-1) producing Escherichia coli isolated in Singapore.
Project description:The emergence of colistin resistance in carbapenem-resistant and extended-spectrum ß-lactamase (ESBL)-producing bacteria is a significant threat to human health, and new treatment strategies are urgently required. Here we investigated the ability of the safe-for-human use ionophore PBT2 to restore antibiotic sensitivity in several polymyxin-resistant, ESBL-producing, carbapenem resistant Gram-negative human pathogens. PBT2 was observed to resensitize Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas aeruginosa to last-resort polymyxin class antibiotics, including a ‘next generation’ polymyxin derivative, FADDI-287. To gain additional insight into the potential mechanism of action of PBT2, we analyzed the transcriptome of K. pneumoniae and E. coli in the presence of sub-inhibitory concentrations of PBT2. Treatment with PBT2 was associated with multiple stress responses in both K. pneumoniae and E. coli. Significant changes in the transcription of transition metal ion homeostasis genes were observed in both strains.
2019-06-13 | GSE132637 | GEO
Project description:Simultaneous hospital outbreaks of New Delhi metallo-beta-lactamase-producing Enterobacterales unraveled using Whole Genome Sequencing.
