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Single cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation

ABSTRACT: Single cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation

PROVIDER: PRJNA506043 | ENA |

REPOSITORIES: ENA

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Single cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation

Project description:Cardiac maturation lays the foundation for postnatal heart development and disease, yet little is known about the contributions of the microenvironment to cardiomyocyte maturation. By integrating single-cell RNA-sequencing data of mouse hearts at multiple postnatal stages, we construct cellular interactomes and regulatory signaling networks. Here we report switching of fibroblast subtypes from a neonatal to adult state and this drives cardiomyocyte maturation. Molecular and functional maturation of neonatal mouse cardiomyocytes and human embryonic stem cell-derived cardiomyocytes are considerably enhanced upon coculture with corresponding adult cardiac fibroblasts. Further, single-cell analysis of in vivo and in vitro cardiomyocyte maturation trajectories identify highly conserved signaling pathways, pharmacological targeting of which substantially delays cardiomyocyte maturation in postnatal hearts, and markedly enhances cardiomyocyte proliferation and improves cardiac function in infarcted hearts. Together, we identify cardiac fibroblasts as a key constituent in the microenvironment promoting cardiomyocyte maturation, providing insights into how the manipulation of cardiomyocyte maturity may impact on disease development and regeneration.

2020-04-20 | GSE122706 | GEO

AP-1 activation mediates postnatal cardiomyocyte maturation

Project description:Mammalian cardiomyocytes rapidly mature after birth, with hallmarks such as cell-cycle exit, binucleation, and metabolic switch to oxidative phosphorylation of lipids. The causes and transcriptional programs regulating cardiomyocyte maturation are not fully understood yet. Thus, we performed single cell RNA-seq of neonatal and postnatal day 7 rat hearts to identify the key factors for this process and found AP-1 as a key factor to regulate cardiomyocyte maturation. To find the mechanism of AP-1 during cardiomyocyte maturation, we performed RNA-seq analysis of neonatal rat ventricular cardiomyocytes and found Ap-1 promote cardiomyocyte maturation by regulating cardiomyocyte metabolism.

2021-12-01 | GSE182422 | GEO

Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.

Project description: Not available

| S-EPMC7244751 | biostudies-literature

Neonatal murine cardiomyocyte transcriptome

Project description:Neonatal murine cardiomyocyte transcriptome

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Effect of cardiomyocyte-specific deletion of glucocorticoid receptor on gene expression of cultured neonatal cardiomyocytes

Project description:To investigate the role of the glucocorticoid receptor (GR) in the regulation of cardiomyocyte maturation and proliferation, we established a cardiomyocyte-specific GR knock-out (GR-cKO) mouse model by Cre-Lox technology. We thus performed gene expression profiling analysis using data obtained from RNA-seq of cardiomyocytes isolated from GR-cKO and control mouse models at neonatal stage and cultured in vitro. Our analyses unveiled a role for GR in regulating gene networks related to the energetic metabolism, which in turn may impact on cardiomyocyte proliferative and regenerative ability.

2022-05-16 | GSE202968 | GEO

Neonatal murine cardiomyocyte transcriptome

Project description:Cardiomyocyte poly(A) RNA was sequenced from purified bulk cardiomyocytes collected from one male and female murine heart at postnatal day 2 (P2). Neonatal cardiomyocytes were isolated and purified (96% cardiomyocytes at P2) by Langendorff retrograde perfusion and immunomagnetic cell separation, respectively. We found evidence of sexual dimorphism with 9 differentially expressed genes (FDR<0.05) encoded on XY chromosomes in this RNA-Seq dataset.

2022-04-30 | GSE193137 | GEO

Isolated cardiomyocyte and cardiac fibroblast expression analysis

Project description:Some cell type-specific gene expression is maintained in the maturation of cardiomyocytes, where DNA hypomethylation of gene body regions of a set of specific genes. We used microarrays to detail the global gene expression program underlying the maintenance of cardiomyocyte function and maturation and compared it with DNA methylation status. Cardiomyocytes and cardiac fibroblasts were carefully isolated from neonatal and adult hearts and used fresh for the analysis.

2012-07-05 | E-GEOD-39039 | biostudies-arrayexpress

Neonatal Heart Maturation (NHM) SuperSeries GSE85728

Project description:Neonatal Heart Maturation (NHM) SuperSeries GSE85728

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AP-1 activation mediates postnatal cardiomyocyte maturation

Project description:AP-1 activation mediates postnatal cardiomyocyte maturation

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Transcription profiling of mouse neonatal mouse cardiomyocyte to investigate the effect of LAMR1-TP1 - INCOMPLETE DATASET

Project description:Infected neonatal mouse cardiomyocyte; COMMENT: Submitter has not provided GEO with full dataset as described in Nat Genet. 2004 Feb;36(2):123-30.

2008-06-16 | E-GEOD-927 | biostudies-arrayexpress

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