Genomewide binding analysis reveals ANAC060 directly represses sugar induced ABI5 transcription in Arabidopsis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA507756 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:p53 directly represses human LINE1 transposons
| PRJNA667740 | ENA
Project description:This SuperSeries is composed of the SubSeries listed below.
2020-11-01 | GSE123170 | GEO
Project description:Genomewide identification of ANAC060 binding targets
| PRJNA507758 | ENA
Project description:Purpose: we performed transcriptome analysis of the wild type and anac060 mutant under glucose treatment to investigated the role of ANAC060 in glucose signalling. Methods: Agilent Arabidopsis 3.0 Oligo Microarray kit which contained nearly 40,000 features representing 28,500 genes was use to investigate of transcriptome of wild type and anac060 mutant under glucose treatment and sorbitol control. Results: By analyzing the transcriptome data, we found that ANAC060 attenuate the glucose signaling and that ANAC060 plays an important role in maintaining the Endogenous stable state of Arabidopsis thaliana in the glucose signaling pathway. The microarray analysis identified 8350 altered genes between different material and treatment combinations. Cluster analysis revealed that ANAC060 attenuates the sugar regulated gene expression. Conclusions: our results demonstrated that ANAC060 directly bind to the promoter of ABI5 and repressed the sugar induced transcription of ABI5, which contributed to render sugar insensitivity.
2020-11-01 | GSE123123 | GEO
Project description:Genomewide mapping of Trim33 binding in Th17 cells
| PRJNA464365 | ENA
Project description:Feeding on high sugar diet reprograms metabolism, partially mediated through a gene regulatory network controlled by intracellular sugar sensor Mondo/ChREBP-Mlx. Sugar-induced gene expression changes include activation of genes encoding enzymes of glycolysis, pentose phosphate pathways, and de novo lipogenesis, collectively driving the flux of sugar-derived carbon into triacylglycerides. These sugar-induced metabolic changes are likely to trigger compensatory responses to avoid depletion of necessary metabolites, but they remain poorly characterized. Through a global temporal clustering analysis of sugar-induced gene expression in Drosophila we identify new gene expression programs controlled by sugar feeding. These include rapid and transient downregulation of ribosome biogenesis genes, known targets of transcription factor Myc. Through in silico motif predictions and experimental validation, we identify transcriptional repressor Clockwork orange (CWO) as a mediator of this response. CWO expression is directly activated by Mondo-Mlx and it counteracts Myc through repression of its expression as well as through binding to overlapping regulatory regions of ribosome biogenesis genes. Loss of CWO function leads to depletion of UDP-GlcNAc pools and impaired viability on high sugar diet. Analysis of CWO orthologs, BHLHE40 and BHLHE41, in mouse hepatocytes revealed a conserved role for BHLHE41 in repressing ribosome biogenesis genes. Collectively, our data uncover a gene regulatory circuit balancing the activities of lipid and protein biosynthesis to maintain homeostasis during high sugar feeding.
2023-06-01 | GSE207197 | GEO
Project description:Feeding on high sugar diet reprograms metabolism, partially mediated through a gene regulatory network controlled by intracellular sugar sensor Mondo/ChREBP-Mlx. Sugar-induced gene expression changes include activation of genes encoding enzymes of glycolysis, pentose phosphate pathways, and de novo lipogenesis, collectively driving the flux of sugar-derived carbon into triacylglycerides. These sugar-induced metabolic changes are likely to trigger compensatory responses to avoid depletion of necessary metabolites, but they remain poorly characterized. Through a global temporal clustering analysis of sugar-induced gene expression in Drosophila we identify new gene expression programs controlled by sugar feeding. These include rapid and transient downregulation of ribosome biogenesis genes, known targets of transcription factor Myc. Through in silico motif predictions and experimental validation, we identify transcriptional repressor Clockwork orange (CWO) as a mediator of this response. CWO expression is directly activated by Mondo-Mlx and it counteracts Myc through repression of its expression as well as through binding to overlapping regulatory regions of ribosome biogenesis genes. Loss of CWO function leads to depletion of UDP-GlcNAc pools and impaired viability on high sugar diet. Analysis of CWO orthologs, BHLHE40 and BHLHE41, in mouse hepatocytes revealed a conserved role for BHLHE41 in repressing ribosome biogenesis genes. Collectively, our data uncover a gene regulatory circuit balancing the activities of lipid and protein biosynthesis to maintain homeostasis during high sugar feeding.
2023-06-01 | GSE207198 | GEO
Project description:Feeding on high sugar diet reprograms metabolism, partially mediated through a gene regulatory network controlled by intracellular sugar sensor Mondo/ChREBP-Mlx. Sugar-induced gene expression changes include activation of genes encoding enzymes of glycolysis, pentose phosphate pathways, and de novo lipogenesis, collectively driving the flux of sugar-derived carbon into triacylglycerides. These sugar-induced metabolic changes are likely to trigger compensatory responses to avoid depletion of necessary metabolites, but they remain poorly characterized. Through a global temporal clustering analysis of sugar-induced gene expression in Drosophila we identify new gene expression programs controlled by sugar feeding. These include rapid and transient downregulation of ribosome biogenesis genes, known targets of transcription factor Myc. Through in silico motif predictions and experimental validation, we identify transcriptional repressor Clockwork orange (CWO) as a mediator of this response. CWO expression is directly activated by Mondo-Mlx and it counteracts Myc through repression of its expression as well as through binding to overlapping regulatory regions of ribosome biogenesis genes. Loss of CWO function leads to depletion of UDP-GlcNAc pools and impaired viability on high sugar diet. Analysis of CWO orthologs, BHLHE40 and BHLHE41, in mouse hepatocytes revealed a conserved role for BHLHE41 in repressing ribosome biogenesis genes. Collectively, our data uncover a gene regulatory circuit balancing the activities of lipid and protein biosynthesis to maintain homeostasis during high sugar feeding.
2023-06-01 | GSE207196 | GEO
Project description:Genomewide methylation profiles in coronary artery ectasia patients and matched healthy controls.
2017-12-21 | GSE87016 | GEO
Project description:Project : Sugar-Auxine_Rose ngs2017_11_rosex2-auxin sugar antagonist effect on budburst Study of the antagonistic effect between sugar and auxin on the bud burst of the axillary bud of the rose bush - Identification of the molecular network involved in the antagonistic effect between sugar and auxin on the budburst of the vegetative budworm of the rose bush
2021-06-21 | GSE123298 | GEO