Ontology highlight
ABSTRACT:
PROVIDER: PRJNA508937 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Draft genome of the Symbiodiniaceae Durusdinium trenchii
| PRJDB10306 | ENA
Project description:Samples are from a screening experiment on 5 laboratory cultures of the coral endosymbiont: Symbiodiniaceae (Symbiodinium linuchae, Breviolum psygmophilum, Durusdinium trenchii, Effrenium voratum and Fugacium kawagutii). Samples are also from a thermal stress experiment (increased temperatures from 26 to 32 degrees C) carried out on Durusdinium trenchii and Cladocopium goreaui, two common coral endosymbionts on the Great Barrier Reef. All samples were collected on Markes Tenax TA thermal desorption tubes.
Lawson, C.A., Possell, M., Seymour, J.R., Raina, J.B. and Suggett, D.J., 2019. Coral endosymbionts (Symbiodiniaceae) emit species-specific volatilomes that shift when exposed to thermal stress. Scientific reports, 9(1), pp.1-11.
2019-10-08 | MSV000084436 | MassIVE
Project description:Cultures of three Symbiodiniaceae genera (Breviolum minutum, Durusdinium trenchii, Cladocopium sp.) were exposed to temperatures increasing from 26C to 32C over three days, followed by one week at 32C. Samples for proteome analysis were taken from controls (26C), at the end of the temperature ramp, and after one week at 32C. The three species have been submitted to PRIDE individually. Manuscript in preparation.
2022-02-23 | PXD025051 | Pride
Project description:Cultures of three Symbiodiniaceae genera (Breviolum minutum, Durusdinium trenchii, Cladocopium sp.) were exposed to temperatures increasing from 26C to 32C over three days, followed by one week at 32C. Samples for proteome analysis were taken from controls (26C), at the end of the temperature ramp, and after one week at 32C. The three species have been submitted to PRIDE individually. Manuscript in preparation.
2022-02-23 | PXD025080 | Pride
Project description:Cultures of three Symbiodiniaceae genera (Breviolum minutum, Durusdinium trenchii, Cladocopium sp.) were exposed to temperatures increasing from 26C to 32C over three days, followed by one week at 32C. Samples for proteome analysis were taken from controls (26C), at the end of the temperature ramp, and after one week at 32C. The three species have been submitted to PRIDE individually. Manuscript in preparation.
2022-02-23 | PXD025050 | Pride
Project description:Transcriptional responses to heat stress were assayed in early life-history stages of 11 crosses between and amongst Acropora tenuis colonies originating from reefs along the Great Barrier Reef. We identified a single nucleotide polymorphism outlier (Fst=0.89) between populations in the unannotated gene Acropora25324, which exhibited constitutively higher gene expression in populations with dams originating from Curd reef, a far north, warm adapted inshore reef, suggesting an important role of this gene in adaptation to warmer environments. Further, juveniles exposed to heat and in symbiosis with heat-evolved Symbiodiniaceae displayed intermediate transcriptional responses between its progenitor taxa (Cladocopium goreaui) and the more stress tolerant Durusdinium trenchii, indicating that the development of heat tolerance acquisition is potentially a conserved evolutionary process in Symbiodiniaceae. These findings reveal the underlying mechanisms, and for the first time, their relative contribution, of coral responses to climate change and provide a foundation for optimizing conservation methods like assistant gene flow.
2022-07-08 | GSE176051 | GEO
Project description:Cellular mechanisms responsible for the regulation of nutrient exchange, immune responses, and symbiont population growth in the cnidarian-dinoflagellate symbiosis are poorly resolved, particularly with respect to the dinoflagellate symbiont. Here, we characterised proteomic changes in the native symbiont Breviolum minutum during colonisation of its host sea anemone Exaiptasia diaphana (‘Aiptasia’). We also compared the proteome of this native symbiont in the established symbiotic state with that of a non-native symbiont, Durusdinium trenchii. The onset of symbiosis between Aiptasia and B. minutum induced increased accumulation of symbiont proteins associated with acquisition of inorganic carbon and photosynthesis, nitrogen metabolism, micro- and macronutrient starvation, suppression of the host immune responses, tolerance to low pH, and management of oxidative stress. Such responses are consistent with a functional, persistent symbiosis. In contrast, D. trenchii predominantly showed elevated levels of immunosuppressive proteins, consistent with the view that this symbiont is an opportunist that forms a less beneficial, less well-integrated symbiosis with this model anemone. By adding this analyses of the symbiont proteins to the already known responses of the host proteome, our results provide a more holistic view of cellular processes that determine host-symbiont specificity and how differences in symbiont partners, native versus non-native symbionts, may impact the fitness of the cnidarian-dinoflagellate symbiosis in response to thermal stress. This PRIDE entry contains the Durusdinium trenchii data; Breviolum minutum data are uploaded in a separate entry with identical parameters.
2024-01-18 | PXD045587 | Pride
Project description:Cellular mechanisms responsible for the regulation of nutrient exchange, immune responses, and symbiont population growth in the cnidarian-dinoflagellate symbiosis are poorly resolved, particularly with respect to the dinoflagellate symbiont. Here, we characterised proteomic changes in the native symbiont Breviolum minutum during colonisation of its host sea anemone Exaiptasia diaphana (‘Aiptasia’). We also compared the proteome of this native symbiont in the established symbiotic state with that of a non-native symbiont, Durusdinium trenchii. The onset of symbiosis between Aiptasia and B. minutum induced increased accumulation of symbiont proteins associated with acquisition of inorganic carbon and photosynthesis, nitrogen metabolism, micro- and macronutrient starvation, suppression of the host immune responses, tolerance to low pH, and management of oxidative stress. Such responses are consistent with a functional, persistent symbiosis. In contrast, D. trenchii predominantly showed elevated levels of immunosuppressive proteins, consistent with the view that this symbiont is an opportunist that forms a less beneficial, less well-integrated symbiosis with this model anemone. By adding this analyses of the symbiont proteins to the already known responses of the host proteome, our results provide a more holistic view of cellular processes that determine host-symbiont specificity and how differences in symbiont partners, native versus non-native symbionts, may impact the fitness of the cnidarian-dinoflagellate symbiosis in response to thermal stress. This PRIDE entry contains the Breviolum minutum data; Durusdinium trenchii data are uploaded in a separate entry with identical parameters.
2024-01-18 | PXD045585 | Pride
Project description:The primary objective of this prospective observational study is to characterize the gut and oral microbiome as well as the whole blood transcriptome in gastrointestinal cancer patients and correlate these findings with cancer type, treatment efficacy and toxicity. Participants will be recruited from existing clinical sites only, no additional clinical sites are needed.
| 2742033 | ecrin-mdr-crc