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High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [Blood]

ABSTRACT: High-throughput combinatorial indexing enables scalable single-cell chromatin accessibility profiling [Blood]

PROVIDER: PRJNA509252 | ENA |

REPOSITORIES: ENA

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Project description:While recent technical advancements have facilitated the mapping of epigenomes at the single-cell resolution, the throughput and quality of these methods have limited the widespread application of these technologies. Here, we describe a high-throughput platform for single-cell assay for transposase accessible chromatin (scATAC-seq) using droplet microfluidics that yields essential qualities for high-throughput profiling, including improvements in per-cell library complexity and throughput. This approach enables robust cell-typing of complex tissues and reveals a de novo atlas of cellular and epigenomic diversity across many cell types and cell stages.

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