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Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions [Emu-Myc]

ABSTRACT: Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions [Emu-Myc]

PROVIDER: PRJNA509276 | ENA |

REPOSITORIES: ENA

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Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions [Emu-Myc]

Project description:Dendritic cell (DC) maturation is a prerequisite for the induction of adaptive immune responses against pathogens and cancer. Transcription factor (TF) networks control differential aspects of early DC progenitor versus late stage DC cell fate decisions. Here, we identified the TF C/EBPβ as a key regulator for DC maturation and immunogenic functionality under homeostatic and lymphoma-transformed conditions. Gene expression profiles of splenic C/EBPβ-/- DCs showed a strong downregulation of E2F cell cycle target genes, whereas signatures of maturation were enriched. In accordance with E2F1 being a negative regulator of DC maturation, C/EBPβ-/- bone marrow-derived DCs matured much faster enabling them to strongly activate T cells. Conversely, the E2F transcriptional pathways were upregulated in lymphoma-exposed DCs and DC maturation was impaired. Pharmacological blockade of C/EBPβ/mTOR signaling in human DCs abrogated their pro-tumorigenic function in B-cell lymphoma co-cultures. Thus, C/EBPβ plays a unique role in DC maturation and functionality and emerges as a key factor of the microenvironment promoting lymphomagenesis.

2020-10-20 | GSE123592 | GEO

Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions [CEBPbetaKO]

2020-10-20 | GSE123591 | GEO

Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions

Project description:Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions

| PRJNA509273 | ENA

Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions [CEBPbetaKO]

Project description:Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions [CEBPbetaKO]

| PRJNA509277 | ENA

Transcription factor C/EBPβ orchestrates DC maturation and functionality under homeostatic and malignant conditions

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-10-20 | GSE123593 | GEO

The transcription factor C/EBPβ orchestrates dendritic cell maturation and functionality under homeostatic and malignant conditions.

Project description: Not available

| S-EPMC7584915 | biostudies-literature

Genome-wide C/EBPβ traget genes in C/EBPβ-overexpressed HL-60 cells.

Project description:To characterize genome-wide C/EBPβ traget genes in C/EBPβ-overexpressed HL-60 cells by ChIP-seq.

2018-06-13 | GSE100486 | GEO

C/EBPβ deficiency reshapes microglial gene expression

Project description:CCAAT/enhancer binding protein β (C/EBPβ) is a transcription factor that regulates the expression of important pro-inflammatory genes in microglia. Mice deficient for C/EBPβ show protection against excitotoxic and ischemic CNS damage but the involvement of the various C/EBPβ expressing cell types in this neuroprotective effect is not solved. Since C/EBPβ-deficient microglia show attenuated neurotoxicity in culture we hypothesized that specific C/EBPβ deficiency in microglia could be neuroprotective in vivo. In this study we have tested this hypothesis by generating mice with myeloid C/EBPβ deficiency. Mice with myeloid C/EBPβ deficiency were generated by crossing LysMCre and C/EBPβfl/fl mice . Primary microglial cultures from C/EBPβfl/fl (named here as WT) and LysMCre-C/EBPβfl/fl (named here as KO) mice were treated with lipopolysaccharide ± interferon γ (IFNγ) for 6 h and gene expression was analyzed by RNA sequencing. LysMCre-C/EBPβfl/fl mice showed an efficiency of C/EBPβ deletion of 100% in cultured microglia. Transcriptomic analysis of C/EBPβ-deficient primary microglia revealed C/EBPβ-dependent expression of 1068 genes, significantly enriched in inflammatory and innate immune responses GO terms. This study provides new data that support a central role for C/EBPβ in the biology of activated microglia.

2017-06-13 | GSE90046 | GEO

Identification of PRDM15 Cistrome in Emu-Myc Lymphoma cells

Project description:Genome-wide binding targets of PRDM15 were identified in Emu-Myc Lymphomas

2019-07-04 | GSE116905 | GEO

DNA microarray analysis of leukemic B cells from Emu-TCL1 and Emu-TCL1-p66ShcKO mice

Project description:To assess the contribution of p66Shc deficiency to the pathogenesis of CLL we introduced a p66Shc null allele into the Emu-TCL1 mouse model of human CLL. The impact of homozygous p66Shc deletion on the pattern of gene expression in leukemic cells from the two mouse strains was compared by DNA microarray, highlighting gene candidates that were subsequently validated and assessed functionally in vitro and in vivo.

2020-11-05 | E-MTAB-9761 | biostudies-arrayexpress

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