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Difference of DNA methylation and Chromatin accessibility of J82 SP cell and J82 NSP cell

ABSTRACT: Difference of DNA methylation and Chromatin accessibility of J82 SP cell and J82 NSP cell

PROVIDER: PRJNA509376 | ENA |

REPOSITORIES: ENA

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Difference of DNA methylation and Chromatin accessibility of J82 SP cell and J82 NSP cell

Project description:Subpopulations of highly tumorigenic, drug resistant cancer stem-like cells play a key role in cancer recurrence and progression. Surprisingly, these aggressive cells can arise repeatedly de novo from bulk tumor cells independently of mutational events. We investigated whether transition to and from a cancer stem-like state is associated with epigenetic alterations, such as DNA methylation and chromatin accessibility. By Hoechst dye staining and FACS analysis, side population (SP) and non-side population cells were sorted from J82 cells. AcceSssIble assay by Infinium EPIC BeadArray platform was used to identify potential epigenetic driver genes in the process of SP /NSP phenotype plasticity. Genes with differential accessible promoter regions were overlapped with genes differentially expressed by HuEx array analysis. Oncomine and Basespace were adopted to explore the clinical relevance of the target genes.

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Difference of DNA methylation and Chromatin accessibility of T24 SP cell and T24 NSP cell

Project description:Difference of DNA methylation and Chromatin accessibility of T24 SP cell and T24 NSP cell

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Difference of DNA methylation and Chromatin accessibility of T24 SP cell and T24 NSP cell

Project description:Difference of DNA methylation and Chromatin accessibility of T24 SP cell and T24 NSP cell

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J82 human bladder cell line treated with frankincense oil

Project description:Frankincense oil is prepared from aromatic hardened wood resin obtained by tapping Boswellia trees. For thousands of years, it has been important both socially and economically as an ingredient in incense and perfumes. Frankincense oil is a botanical oil distillate made from fermented plants that contains boswellic acid, a component known to have anti-neoplastic properties. We evaluated frankincense oil-induced cytotoxicity in bladder cancer cells. With a window of concentration, frankincense oil suppressed cell viability and induced cytotoxicity in bladder transitional carcinoma J82 cells but not normal bladder urothelial UROtsa cells immortalized with SV40 large T antigen. However, frankincense oil-induced J82 cell death did not result in DNA fragmentation. Microarray and bioinformatics analysis confirmed that frankincense oil activated cell cycle arrest, suppressed cell proliferation, and activated apoptosis in J82 cells through a series of potential pathways. These finding suggest that bladder cancer can be treated through intravesical administration of pharmaceutical agents similar to direct application on melanoma. 2E05 J82 cells were untreated or treated with a 1/1000 dilution of frankincense oil for 0.5, 1, 2, or 3 hours prior to RNA extraction.

2009-02-09 | E-GEOD-14002 | biostudies-arrayexpress

Expression data from J82 side population and non-side population cells

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J82 human bladder cell line treated with frankincense oil

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RNA-Seq of circCDYL knockdown (KD) samples in HepG2, J82, and UMUC3 cells and of GRWD1, IGF2BP1, and IGF2BP2 knockdown (KD) samples in J82 and UMUC3 cells

Project description:We profile gene expression upon circCDYL KD in HepG2 cells and in two bladder cancer cell lines J82 and UMUC3 as well as upon knockdown of the RNA binding proteins (RBP) GRWD1, IGF2BP1, and IGF2BP2 in J82 and UMUC3

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RNA-Seq of circHIPK3 knockdown (KD) samples in UMUC3 and J82

Project description:We profile gene expression upon circHIPK3 KD in two bladder cancer cell lines UMUC3 and J82

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Expression data from human immortalized urothelial cell SV-HUC-1 and four Bladder cancer cells (5637, UMUC3, T24, and J82)

Project description:To uncover the dysregulated genes in BCa, we performed the RNA-seq to detect the gene expression levels in four BCa cells (5637, UMUC3, T24, and J82) and human immortalized urothelial cell SV-HUC-1. To search for the differentially expressed genes of for cell lines (5637, J82, UMUC3, and T24) compared to the normal cell line SV-HUC-1.

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Expression data from human Bladder cancer cell line J82 with control or overexpression of SQLE

Project description:Several reports showed that SQLE was upregulated in some types of cancer, and it is essential for cancer development. Herein, to uncover the role of SQLE in BCa, we performed the RNA-seq to detect the gene expression levels in BCa cell line J82 with overexpression of SQLE.

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