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In vivo RNA editing of point mutations via RNA-guided adenosine deaminases

ABSTRACT: In vivo RNA editing of point mutations via RNA-guided adenosine deaminases

PROVIDER: PRJNA510252 | ENA |

REPOSITORIES: ENA

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In vivo RNA editing of point mutations via RNA-guided adenosine deaminases

Project description:We investigated the specificity profiles of a variety of RNA guided adenosine deaminases while exploring roles of NLS/NES and hyperactive mutants via analysis of the transcriptome-wide off-target A->G editing effected by these tools. To this end, HEK 293T cells were transfected with each construct and analyzed by RNA-seq. Untransfected cells were included as controls. From each sample, we collected ~40 million uniquely aligned sequencing reads. We then used Fisher’s exact test to quantify significant changes in A->G editing yields, relative to untransfected cells, at each reference adenosine site having sufficient read coverage. The number of sites with at least one A->G editing event detected in any of the samples was computed.

2018-12-17 | GSE123905 | GEO