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Genome-wide DNA methylation analysis in primary antiphospholipid syndrome neutrophils

ABSTRACT: Genome-wide DNA methylation analysis in primary antiphospholipid syndrome neutrophils

PROVIDER: PRJNA512512 | ENA |

REPOSITORIES: ENA

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Genome-wide DNA methylation analysis in primary antiphospholipid syndrome neutrophils

Project description:Antiphospholipid syndrome (APS) is a systemic autoimmune disease characterized by thromboembolic events and pregnancy loss. We sought to characterize the DNA methylation profile of primary APS in comparison to healthy controls and individuals with SLE.

2019-01-02 | GSE124565 | GEO

RNA-Seq in neutrophils from Antiphospholipid Syndrome

Project description:Antiphospholipid antibodies are a leading cause of thrombosis. To further elucidate the role of neutrophils in APS, we performed a comprehensive transcriptome analysis using the total RNA of neutrophils isolated from 9 patients with primary APS and 9 age, sex and ethnicity-matched healthy controls using next-generation RNA-seq.

2017-11-15 | GSE102215 | GEO

RNA sequencing of primary Sjögren’s syndrome neutrophils

Project description:RNA sequencing of primary Sjögren’s syndrome neutrophils

| PRJNA799777 | ENA

Genome-wide DNA methylation analysis in primary antiphospholipid syndrome neutrophils.

Project description: Not available

| S-EPMC6413498 | biostudies-literature

RNA sequencing of primary Sjögren’s syndrome neutrophils

Project description:Objective Neutrophils and aberrant NETosis have been implicated in the pathogenesis of diverse autoimmune diseases, however, their roles in primary Sjögren’s syndrome (pSS) remain unclear. We aimed to reveal the potential roles of neutrophils and neutrophil extracellular traps (NETs) in this study. Methods pSS patients were enrolled according to the corresponding diagnostic criteria. NETosis markers were measured in plasma and small salivary gland using ELISA and immunofluorescence. The gene signatures of neutrophils were assessed by RNA-Seq and RT-PCR. Reactive oxygen species (ROS), mitochondrial ROS (mitoROS) production and JC-1 was measured by flow cytometry. Results NETosis markers including cell free-DNA (cf-DNA), myeloperoxidase (MPO) in plasma and small salivary gland from pSS patients were significantly higher than healthy controls (HCs) and were associated with disease activity. RNA sequencing and RT-qPCR revealed activated Type I IFN signaling pathway and higher expression of type I interferon related genes in pSS neutrophils. Further stimulating with IFN-α 2a in vitro significantly induced ROS production and JC-1 monomer percentage in pSS neutrophils. Conclusions Our data suggest the involvement of neutrophils and enhanced NETosis in pSS patients. Further mechanism study in vitro revealed that type I IFN activation in pSS neutrophils led to mitochondrial damage and related ROS production which finally result in the generation of NETs.

2022-01-25 | GSE194234 | GEO

Gene profiling reveals specific molecular pathways in the pathogenesis of atherothrombosis in Antiphospholipid syndrome, Systemic Lupus Erythematosus and Lupus with Antiphospholipid Syndrome

2014-03-12 | GSE50395 | GEO

Transcriptome profiling of PBMCs from primary antiphospholipid syndrome and systemic lupus erythematosus patients and healthy controls

Project description:explore the transcriptome profiles of PBMCs derived from individuals with Primary antiphospholipid syndrome (PAPS) and compared<br>them with those of healthy individuals. Also compare the transcriptome profiles of the closely related Systemic lupus Erythematosus autoimmune disorder with the same profiles from healthy individuals

2008-06-27 | E-MEXP-1635 | biostudies-arrayexpress

Genome-wide DNA methylation patterns in monocyte from patients with primary Sjögren’s syndrome

Project description:To reveal the role of DNA methylation in peripheral monocytes (Mo) of primary Sjögren’s syndrome (pSS) patients, monocyte DNAs from 11 pSS patients and 5 matched controls were analyzed by methylation microarray. In total, we identified 1977 hypomethylated and 842 hypermethylated differentially methylated positions (DMPs) in Mo from pSS patients compared to healthy controls.

2021-06-16 | GSE146116 | GEO

Homo sapiens

Project description:RNA-Seq in neutrophils from Antiphospholipid Syndrome

| PRJNA397036 | ENA

Gene profiling reveals specific molecular pathways in the pathogenesis of atherothrombosis in Antiphospholipid syndrome, Systemic Lupus Erythematosus and Lupus with Antiphospholipid Syndrome

Project description:The present gene expression array study of comparative gene profile in monocytes from patients with primary Antiphospholipid Syndrome, Systemic Lupus Erythematosus and Lupus with Antiphospholipid Syndrome demonstrates that the gene expression profiling allows the segregation of these highly related autoimmune diseases, with specific signatures explaining the pro-atherosclerotic, pro-thrombotic and inflammatory changes. One hundred and twenty six patients, forty one with APS, thirty one with SAPS and fifty four with SLE, as well as sixty one healthy donors were included in the study. Monocytes were purified from peripheral blood samples (non-monocytes depleting kit, Miltenyi Biotech, Bergisch Galdbach, Germany). Total RNA from monocytes was extracted using TRIzol reagent. RNA quality control was performed in a 2100 Bioanalyzer. Complementary RNAs from 3 APS patients, 3 SAPS patients, 3 SLE patients, and 3 healthy donors were prepared for hybridization in an Agilent G4112F platform (Whole human Genome microarray 44K) using the One-color gene expression system (Agilent technologies).

2014-03-12 | E-GEOD-50395 | biostudies-arrayexpress

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