Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq on Fixed and Permeabilized Plasma cells

ABSTRACT: RNA-seq on Fixed and Permeabilized Plasma cells

PROVIDER: PRJNA512528 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

RNA-seq on Fixed and Permeabilized Plasma cells

Project description:Here we developed a protocol to extract total RNA from fixed and permeabilized cells that were intracellularly stained and FACS isolated. We applied these techniques to derive the transcriptome of influenza- and BCR isotype-specific antibody secreting cells or plasma cells. These data reveal that ASC isotypes have more distinct transcriptional signatures than previously appreciated.

2019-08-14 | GSE124578 | GEO

FD-seq: Droplet-based RNA sequencing of fixed single cells

Project description:FD-seq: Droplet-based RNA sequencing of fixed single cells

| PRJNA659731 | ENA

Chromatin accessibility and gene expression maps of Plasma Cells and Myeloma Plasma Cells [RNA-seq]

Project description:Chromatin accessibility and gene expression maps of Plasma Cells and Myeloma Plasma Cells [RNA-seq]

| PRJNA642175 | ENA

Transcriptional profiling of mouse plasma cells (population RNA-Seq)

Project description:Transcriptional profiling of mouse plasma cells (population RNA-Seq)

| PRJNA476324 | ENA

RNA-seq analysis of hepatocellular carcinoma plasma

Project description:RNA-seq analysis of hepatocellular carcinoma plasma

| PRJNA510411 | ENA

RNA-Seq Analysis

Project description:RNA-Seq analysis carried out in three different backcrosses based on Iberian breed with Landrace, Pietrain and Duroc breeds

2017-10-31 | PRJEB23068 | EVA

Analysis of RNA quantitation in formaldehyde-fixed cells

Project description:Fluorescence-activated cell sorting (FACS) is a sensitive and valuable technique to characterize cellular subpopulations and great advances have been made using this approach. Cells are often fixed with formaldehyde prior to the sorting process to preserve cell morphology and maintain the expression of surface molecules, as well as to ensure safety in the sorting of infected cells. It is widely recognized that formaldehyde fixation alters RNA and DNA structure and integrity, thus analyzing gene expression in these cells has been difficult. We therefore examined the effects of formaldehyde fixation on the stability and quantitation of nucleic acids in cell lines, primary leukocytes and also cells isolated from SIV-infected pigtailed macaques. We developed a method to extract RNA from fixed cells that yielded the same amount of RNA as our common method of RNA isolation from fresh cells. Quantitation of RNA by RT-qPCR in fixed cells was not always comparable with that in unfixed cells. In comparison, when RNA was measured by the probe-based NanoString system, there was no significant difference in RNA quantitation. In addition, we demonstrated that quantitation of proviral DNA in fixed cells by qPCR is comparable to that in unfixed cells when normalized by a single-copy cellular gene. These results provide a systematic procedure to quantitate gene expression in cells that have been fixed with formaldehyde and sorted by FACS.

2013-09-09 | GSE50690 | GEO

RNA-seq analysis of liver cancer patients' plasma

Project description:RNA-seq analysis of liver cancer patients' plasma

| PRJNA598835 | ENA

Chromatin accessibility and gene expression maps of Plasma Cells and Myeloma Plasma Cells [ATAC-seq]

Project description:Chromatin accessibility and gene expression maps of Plasma Cells and Myeloma Plasma Cells [ATAC-seq]

| PRJNA642174 | ENA

Transcriptional profiling of mouse plasma cells (scRNA-Seq)

Project description:Transcriptional profiling of mouse plasma cells (scRNA-Seq)

| PRJNA476323 | ENA