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Single cell multi-omics analysis reveals novel roles for DNA methylation in sensory neuron injury responses

ABSTRACT: Single cell multi-omics analysis reveals novel roles for DNA methylation in sensory neuron injury responses

PROVIDER: PRJNA513289 | ENA |

REPOSITORIES: ENA

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Single cell multi-omics analysis reveals novel roles for DNA methylation in sensory neuron injury responses

Project description:We used single cell multi-omics method to simultaneously profiled both DNA methylome and mRNA transcriptome from single DRG sensory neurons under either control or peripheral nerve injury condition.

2019-10-24 | GSE124728 | GEO

Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury

Project description:Primary somatosensory neurons specialize in transmitting distinct types of sensory information through differences in cell size, myelination, and the expression of various receptors and ion channels, which together define their transcriptional and functional identity. By profiling sensory ganglia at single-cell resolution, we find that the different somatosensory neuronal subtypes undergo a similar transcriptional response to peripheral nerve injury that both promotes axonal regeneration and suppresses cell identity. This transcriptional reprogramming, which is not observed in non-neuronal cells from sensory ganglia, resolves over a similar time course as target reinnervation and is associated with the restoration of original cell identity. Injury-induced transcriptional reprogramming requires ATF3, a transcription factor which is induced rapidly after injury and necessary for axonal regeneration and functional recovery. Our findings suggest that the transcription factors induced early after peripheral sensory neuron injury promote their transcriptional and functional metamorphosis, analogous to their known roles in reprogramming cell fate.

2020-07-22 | GSE154659 | GEO

Peripheral Sensory Neuron EC-tagging experiments

Project description:Peripheral Sensory Neuron EC-tagging experiments

| PRJNA663560 | ENA

Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury

Project description:Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury

| PRJNA646966 | ENA

Profiling Kif5A RNA targets in sensory neuron axons

Project description:Profiling Kif5A RNA targets in sensory neuron axons

| PRJNA595899 | ENA

Transcriptome analysis of metronidazole-induced sensory neuron ablation in zebrafish

Project description:Damage to and/or loss of sensory neurons can result in debilitating neuropathies that often have a dramatic impact on quality of life. The cellular mechanisms involved in the response of neurons and glia to such pathological insults are poorly understood. Investigation has shown that peripheral glia play critical roles in both the degenerative and regenerative processes that are involved in the responses to peripheral nerve damage. The vast majority of studies have focused primarily on myelinating Schwann cells], with the result that very little is known regarding how the non-myelinating glia that ensheath axons and neuronal somas respond to nerve damage. This is a significant knowledge gap, given that over 80% of cutaneous fibers are unmyelinated, that they transduce such important modalities as itch, pain, temperature, touch and pressure, and that they are affected in many prevalent peripheral neuropathies. It is the goal of this study to shed light on the genetic programs involved in the responses of non-myelinating glia roles to nerve degeneration. We utilized RNA-seq to identify genes that were differentially expressed in the larval head during the process of sensory neuron ablation and axon degeneration in both wild-type larvae and in larvae that do not have peripheral glia (foxd3 mutants) using a selective, conditional approach. Overall, the information regarding differential gene expression in these conditions will provide a basis for further investigation into the cellular processes that underlie pathophysiological responses of neurons and glia to sensory nerve damage.

2017-08-31 | GSE72682 | GEO

Evaluation of Tmem184b dependent gene expression changes during sensory neuron differentiation in vitro

Project description:Our objective was to evaluate the gene expression changes occuring in early sensory neuron development that were lost in the absence of Tmem184b and restored upon its reintroduction into mutant neurons in culture.

2020-07-15 | GSE154435 | GEO

Single-cell RNAseq Reveals Seven Classes of Visceral Sensory Neuron

Project description:Integration of nutritional, microbial and inflammatory events along the gut-brain axis can alter bowel physiology and organism behaviour. The principal neural unit in the bowel encoding these stimuli is the visceral sensory neuron with endings at the mucosa, intramurally and along mesenteric blood vessels. Sensory neurons activate reflex pathways and give rise to conscious sensation, however, the diversity and division of function within these neurons is poorly understood. The identification of signalling pathways contributing to visceral sensation is constrained by the current paucity of molecular markers. Here we overcome these limitations by comprehensive transcriptomic profiling and unsupervised clustering of single colonic sensory neurons revealing 7 classes characterised from both lumbar splanchnic (LSN) and pelvic nerves (PN). We identify and classify neurons based on novel marker genes, confirm translation of patterning to protein expression and show subtype-selective differential agonist activation, describing sensory diversity encompassing all modalities of colonic neuronal sensitivity.

2018-02-27 | GSE102962 | GEO

Silencing of odorant receptor gene expression by G protein M-NM-2M-NM-3 signaling ensures the expression of one odorant receptor per olfactory sensory neuron

Project description:Olfactory sensory neurons express just one out of a possible ~1000 odorant receptor genes, reflecting an exquisite mode of gene regulation. In one model, once an odorant receptor is chosen for expression, other receptor genes are suppressed by a negative feedback mechanism, ensuring a stable functional identity of the sensory neuron for the lifetime of the cell. The signal transduction mechanism subserving odorant receptor gene silencing remains obscure, however. Here we demonstrate in the zebrafish that odorant receptor gene silencing is dependent on receptor activity. Moreover, we show that signaling through G protein M-NM-2M-NM-3 subunits is both necessary and sufficient to suppress the expression of odorant receptor genes, and likely acts through histone methylation to maintain the silenced odorant receptor genes in transcriptionally inactive heterochromatin. These results provide new insights linking receptor activity with the epigenetic mechanisms responsible for ensuring the expression of one odorant receptor per olfactory sensory neuron. Total 6 samples were analyzed-3 controls & 3 samples

2013-12-16 | E-GEOD-53334 | biostudies-arrayexpress

Proprioception is governed by highly diverse and versatile sensory neuron types

Project description:Proprioception is governed by highly diverse and versatile sensory neuron types

| PRJNA657004 | ENA