Project description:Investigation of the genetic diversity of Emiliania huxleyi, genomic DNA from 15 different strains were compared with the genomic DNA of the sequenced E. huxleyi strain CCMP1516. Gephyrocapsa oceanica and Isochrysis galbana as phylogenetic closely related taxa were used as out-groups.
Project description:In order to determine how the bacterial quorum-sensing precursor molecule 2-heptyl-4-quinolone (HHQ) induces cellular senescence in populations of some eukaryotic marine phytoplankton, we examined the transcriptional response of Emiliania huxleyi CCMP2090 to HHQ exposure. Quadruplicate batch cultures were treated with HHQ at three concentrations (1, 10 and 100 ng/ml) and compared to a vehicle control after 24 and 72 hours of exposure. The majority of gene expression changes occurred after 24 hr of exposure in the 10 and 100 ng/ml treatments and many of these changes persisted for 72 hr in the 100 ng/ml treatment. RNAseq analysis revealed that HHQ exposure impacts many aspects of Emiliania huxleyi metabolism including: chlorophyll biosynthesis and light harvesting, vitamin biosynthesis, vesicle trafficking, and general signals related to energy production. Changes in genes involved in intraphase cell cycle check points implicate DNA damage as the primary driver of HHQ-induced cellular senescence.
Project description:Gene expression analysis of Emiliania huxleyi after 6, 12 and 24 hours of viral infection infected with the virus EhV-86 in comparison to an unifected culture.
Project description:We performed single-cell transcriptome analysis (using MARS-seq) for the worldwide-distributed microalga Emiliania huxleyi during infection by its specific coccolithovirus. Our results provide insights into the expression programs and infection strategies of the large virus, and highlight the potential of single-cell RNA-sequencing for microbial eukaryotes in the lab and in the field.
