Project description:To further explore the molecular mechanisms of flowering regulation in pitaya, we used de novo RNA sequencing-based transcriptomic analysis for four stages of pitaya subjected to light induction. We assembled 68113 unigenes in total, of which 29782 unigenes with functional annotations in the Nr database, 20716 annotations in SwissProt, 18088 annotations in KOG, and 11059 annotations in Kegg
Project description:Canker disease caused by Neoscytalidium dimidiatum is the most serious disease that attacks the pitaya industry. One pathogenic fungus, referred to as ND8, was isolated from the wild-type red-fleshed pitaya (Hylocereus polyrhizus) of Hainan Province. Here, we studied mainly the host responses of red-fleshed pitaya (H. polyrhizus) cultivars against N. dimidiatum using Illumina RNA-Seq technology.
Project description:The pathogenic fungus Neoscytalidium dimidiatum (Nd) is the causal agent of pitaya canker and causes significant yield losses. The mechanism by which Nd invades pitaya stems remains largely unknown. Here, quantitative proteomic analysis was employed to investigate pitaya immune responses against Nd infection. A total of 2766 proteins including 244 differentially expressed proteins (DEPs) were identified during the infection cycle. Bioinformatics analysis indicated that the DEPs were associated with photosynthesis, phytohormone activity, reactive oxygen species (ROS) homeostasis and pathogenic defense responses. This study demonstrates that comparative proteomics is an effective strategy for providing new perspective on biotic stress from fungal pathogens in pitaya.
Project description:RNA-seq of wheat lodicules in two higly-chasmogamous (HCH) (Piko and Poezja) and two low-chasmogamy (LCH) (Euforia and KWS Dacanto) varieties at two developmental stages - pre-flowering and early flowering.
Project description:We identified differentially expressed genes in flail mutants linked to the regulation of flowering through stranded RNA-seq. A genomic rescue fragment of FLAIL (pFLAIL:gFLAIL) introduced in flail mutants can complement expression defects and early flowering, consistent with trans-acting effects of the FLAILRNA. We determined the genomic binding regions of FLAIL by ChIRP-seq. FLAIL binding to chromatin regions promotes the expression of selected flowering repressors.
Project description:We identified differentially expressed genes in flail mutants linked to the regulation of flowering through stranded RNA-seq. A genomic rescue fragment of FLAIL (pFLAIL:gFLAIL) introduced in flail mutants can complement expression defects and early flowering, consistent with trans-acting effects of the FLAILRNA. We determined the genomic binding regions of FLAIL by ChIRP-seq. FLAIL binding to chromatin regions promotes the expression of selected flowering repressors.