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Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo (miRNA-Seq)

ABSTRACT: Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo (miRNA-Seq)

PROVIDER: PRJNA516456 | ENA |

REPOSITORIES: ENA

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Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo

Project description:A long-prevailing model has held that the “seed” region (nucleotides 2-8) of a microRNA is typically sufficient to mediate target recognition and repression. However, numerous recent studies, both within the context of defining miRNA/target pairs by direct physical association and by directly assessing this model in vivo in C. elegans have brought this model into question. To test the importance of miRNA 3' pairing in vivo, in a mammalian system, we engineered a mutant murine mir-146a allele in which the 5' half of the mature microRNA retains the sequence of the wild-type mir-146a but the 3ʹ half has been altered to be anti-complementary to the wild-type miR-146a sequence. Mice homozygous or hemizygous for this mutant allele are phenotypically indistinguishable from wild-type controls and do not recapitulate any of the immunopathology previously described for mir-146a-null mice. Our results strongly support the conclusion that 3ʹ pairing is dispensable in the context of the function of a key mammalian microRNA.

2019-02-09 | PXD011413 | Pride

Mir-146a wild-type 3ʹ sequence identity is dispensable for proper innate immune function in vivo (miRNA-Seq)

2019-02-06 | GSE125473 | GEO

Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo (RNA-Seq)

2019-02-06 | GSE121232 | GEO

Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo

Project description:Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo

| PRJNA516453 | ENA

Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo (RNA-Seq)

Project description:Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo (RNA-Seq)

| PRJNA496417 | ENA

MiR-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo.

Project description: Not available

| S-EPMC6379685 | biostudies-literature

Mir-146a wild-type 3' sequence identity is dispensable for proper innate immune function in vivo

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-02-06 | GSE125474 | GEO

miR-146a regulates many lncRNAs in RAS induced senescence [Anti-146a]

Project description:miR-146a is a known anti-inflammatory miRNA. Intringuigly, it is overexpressed in RAS-induced senescent cells which is accompanied with a rich pro-inflammatoy secretpry phenotype. We aim to study possible sponges for miR-146a.

2021-05-11 | GSE150325 | GEO

Gene Regulation in Bob1-/- and miR-146a-/- Murine Pre-B Cells

Project description:5d IL-7-supported bone marrow cultures from C57BL/6 mice deficient for either the transcription factor Bob1 or microRNA-146a Micro-RNAs (miRNAs) are required for many processes during hematopoietic development and activation, including B-cell differentiation. The lymphocyte-specific transcription factor Bob1 is critical for the proper development and function of B cells. Bob1-/- mice are immunodeficient and lack germinal centers. Microarray expression analysis of Bob1-deficient B cells shows numerous up-regulated genes, suggesting that Bob1 may affect targets indirectly via the control of miRNAs. Using a gain-of-function/loss-of-function approach in WEHI-231 and primary pro-B and pre-B cells, we identified miR-146a as a direct target of Bob1. Ttranscriptome-wide analysis of miR-146a-/- B cells shows a number of misregulated genes involved in immune regulation, while analysis of Bob1-/- cells shows increased expression of cell cycle-related genes. These results support previous evidence that Bob1 suppresses proliferation, a previously underappreciated role for Bob1 that is in line with the cancer-prone phenotype of mir-146a-/- mice.

2013-09-07 | GSE44986 | GEO

Gene Regulation in Bob1-/- and miR-146a-/- Murine Pre-B Cells

2013-09-07 | E-GEOD-44986 | biostudies-arrayexpress

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