Project description:The growth factor, TGF-beta can have profound effect on global gene expression changes. Since TGF-beta signaling is not well studied in liver epithelia, RNA-seq analysis was performed to evaluate TGF-beta signaling in cholangiocytes.
Project description:Growth factor, TGF beta can have profound effect on global gene expression changes. Since TGF beta signalling is not well studied in liver epithelia , we decided to do Next gen RNA-seq analysis to look at TGF beta signaling in cholangiocytes
Project description:TGF beta has profound effects on global gene expression changes. To understand the epigenetic changes associated with TGF beta stimulation, we performed ATAC-seq of cholangiocytes to understand the changes in chromatin accessibility associated with TGF beta. We also wanted to determine if H3K9ac played a role in TGF beta induced changes in chromatin accessibility. Therefore, H3K9ac inhibitor CPTH6 was used to treat cells.
Project description:TGF beta has profound effects on global gene expression changes. To understand the epigenetic mechanisms associated with TGF beta stimulation, we performed ChIP-seq of cholangiocytes to understand the changes in histone modifications associated with TGF beta. We choose H3K27ac and H3K9ac, histone modification associated with gene expression. These histone modifications were correlated with SMAD3 occupancy, which is a canonical downstream mediator of TGF beta signaling.
Project description:Global expression profile of human osteoblast treated with recombinant TGF-beta compared to human osteoblast treated with growth media alone Dye-swap design with 6 biological replicates. Three arrays performed with TGF-beta treated samples on channel 1 and media-alone treated on channel 2; three arrays performed with TGF-beta treated samples on channel 2 and media-alone on channel 1.
Project description:Global expression profile of human osteoblast treated with recombinant TGF-beta compared to human osteoblast treated with growth media alone
Project description:TGF-β is a major inducer of epithelial-mesenchymal transition (EMT) during cancer progression, mainly by activating a set of pleiotropic transcription factors and other classes of genes We used microarrays to detail the global programme of gene expression underlying TGF-β-induced EMT and identified distinct classes of TGF-β-regulated lncRNAs during this process.
Project description:CD14+ human immature myeloid cells were treated with TGF-beta for increased periods of time to study the effects of TGF-beta in regulating the myeloid cell lineage and functions.
Project description:Advanced ovarian cancer is the most lethal gynecologic malignancy in the United States. Ovarian cancer cells are known to have diminished response to TGF-beta, but it remains unclear whether TGF-beta can modulate ovarian cancer cell growth in an indirect manner through cancer-associated fibroblasts (CAFs). Using transcriptome profiling analyses on TGF-beta-treated ovarian fibroblasts, we identified a TGF-beta-responsive gene signature in ovarian fibroblasts. Identifying TGF-beta-regulated genes in the ovarian microenvironment helps in understanding the role of TGF-beta in ovarian cancer progression. The human telomerase-immortalized ovarian fibroblast line NOF151 was treated with 5ng/mL of either TGF-beta-1 or TGF-beta-2. Total RNA was isolated from control samples and TGF-beta-treated fibroblasts samples at 48 hours post-treatment, followed by cDNA synthesis, IVT and biotin labeling. Samples were then hybridized onto Affymetrix Human Genome U133 Plus 2.0 microarrays. For each treatment group, three independent samples were prepared for the microarray experiment.