Project description:We report the application of in situ Hi-C technology to 40 colorectal cancer patients and 10 paried-normal tissue to indentify CRC specific changes in 3D chromatin structure. The chromatin contact matrix were generated by the sequencing data and image processing/deep learning-based algorithm was proposed to identify long-range abnormal chromatin interaction patterns in the contact matrix. The tumor specific 3D chromatin sturcutre changes and the enhancer-promoter rewiring mediated by the identified chromatin sturcture changes were analyzed. The complex chromosome-wide rearrangements such as chromothripsis and its effect to 3D chromatin sturucters were also observed.