Ontology highlight
ABSTRACT:
PROVIDER: PRJNA520880 | ENA |
REPOSITORIES: ENA
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Project description:Ramie fibers extracted from the stem barks have been utilized for thousands years in China, and its biosynthetic mechanism is poorly understood. Here, we have characterized and compared the expression profiling of the barks from the top and middle part of stem where the secondary cellular walls (SCWs) of fiber cell have not initiated growth and was thickening, respectively; resulting in 4,520 differential expression (DE) protein-coding transcripts (PCTs), 1,287 DE long noncoding RNAs (lncRNAs), 88 DE miRNAs and 78 DE circular RNAs (circRNAs). Among the 4,520 DE PCTs, 75 were identified to be the homolog of the Arabidopsis SCW-biosynthetic genes. Overexpression of whole_GLEAN_10014861 (a homolog of SND1) in Arabidopsis caused a significant up-regulation in the expression of AtMYB46 and AtMYB83 that are two key regulators for SCW biosynthesis, thus conferring more fiber cells, along with a remarkable thickening in the SCWs of these cells. Bioinformatic prediction revealed 14 of the 75 homolog targeted by 16 noncoding RNAs (including 8 miRNAs and 8 lncRNAs), and two involved in the competing endogenous RNAs networks, indicating a complex regulatory network for the SCW biosynthesis of bast fiber in ramie. This study provides important insights into the bast fiber biosynthesis of ramie.
2019-05-02 | GSE130587 | GEO
Project description:Corchorus olitorius L. and Corchorus capsularis L Raw sequence reads
| PRJNA378897 | ENA
Project description:Cellulosic phloem fibres, also known as bast fibres, find a wide array of applications in industry, for example as eco-friendly substitutes of glass fibres in the manufacture of biocomposites. Bast fibres are cells characterized by a noteworthy length and by a tertiary cell wall (gelatinous or G-layer) mainly composed of crystalline cellulose. During their differentiation, bast fibres undergo specific developmental stages: the cells initially elongate rapidly by intrusive growth, then they cease elongation and start to thicken. In the light of their importance in fostering a bio-economy, it is desirable to widen our knowledge on the molecular mechanisms underlying the differentiation and maturation of bast fibres. The goal of the present study is to provide a transcriptomic close-up of the key events accompanying bast fibre development. The stages going from rapid elongation to cell wall thickening are here studied in textile hemp (Cannabis sativa L.), a fibre crop of great economic importance.
2017-07-10 | GSE94156 | GEO
Project description:Extracellular matrix (ECM) is the first line of defense which is an inimitable organelle that perceives biotic and abiotic stresses and reprograms biological processes of host. It also activates innate immune responses in temporal and spatial manner and acts as physical scaffold that prevents the entry of pathogen and microbes in response to stress and hormonal signals. Stem rot, caused by Macrophomina phaseolina adversely affects fiber production in jute. However, how wall related susceptibility affects ECM proteome remains undetermined in bast fiber crops. In order to develop ECM proteome of C. olitorius var. O-4, ECM fraction was isolated by mechanical disruption, CaCl2 extraction and enrichment. ECM fraction purity was assessed by 1-DE profiling in which the ECM fraction was found to be discrete from the crude extract and supernatant recovered during purification steps. Three biological replicates were performed for gel-based jute ECM proteomic analysis. MS/MS analysis of total extract and supernatants were identified as Rubisco or other chloroplastic proteins which were depleted in consecutive steps of purification depicting high degree of ECM purity. Fourteen bands of ECM enriched fraction were identified as canonical ECM localized proteins, e.g., beta-glucosidase, beta-d xylosidase, peroxidase, glycoside hydrolase and peroxiredoxin, confirming jute ECM protein purity and enrichment.
2024-05-21 | PXD046939 | Pride
Project description:Corchorus capsularis cultivar:Yueyuan5hao Raw sequence reads
| PRJNA636645 | ENA
Project description:Corchorus capsularis cultivar:Yueyuan5hao Raw sequence reads
| PRJNA612936 | ENA
Project description:Corchorus capsularis cultivar:Yueyuan5hao Raw sequence reads
| PRJNA612788 | ENA