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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

ABSTRACT: High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

PROVIDER: PRJNA520989 | ENA |

REPOSITORIES: ENA

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High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune respone and the relationship between serine synthesis pathway and LPS induced innate immune respone Methods: BMDM cells were treated with or without LPS for 6 hours in the pressence of phgdh inhibitor or not. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeqTM 2000. And the differencial mRNA profiles were analyzed. Results: mRNA profiles about 23002 genes were analyzed, and differencial expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of mouse BMDM treated with LPS and phgdh inhibitor, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between serine synthesis pathway and LPS induced innate immune respone

2019-09-23 | GSE126084 | GEO

High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

Project description:High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

| PRJNA758996 | ENA

High-throughput sequencing Analysis of Transcriptomes of depolarized mouse BMDM cell

Project description:Purpose:Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune respone and the relationship between the membrane potential and LPS induced innate immune respone Methods: WT BMDM cells were treated with or without LPS for 6 hours in the pressence of membranepotential depolarization stimuli. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using Illumina HiSeq /Novaseq or MGI2000. And the differencial mRNA profiles were analyzed. Results: mRNA profiles of more than 20000 genes were analyzed, and differencial expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of WT and membrane potential depolarized macrophages, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between membrane potential and LPS induced innate immune respone

2022-04-05 | GSE183067 | GEO

High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

Project description:High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

| PRJNA655678 | ENA

High-throughput sequencing Analysis of mouse BMDM cell Transcriptomes after LPS Challenge

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity.This study was designed to analyze LPS induced innate immune respone and the gene expression in WT and HDAC3 KO BMDMs. Methods: BMDM cells from Hdac3f/f and Lyz2-Cre-Hdac3f/f were treated with or without LPS for 4 hours. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeqTM2000/MiSeq. And the differencial mRNA profiles were analyzed. Results: mRNA profiles were analyzed, and differencial expression profiles were compared . Conclusions: Our study demostrated the transcriptional profiles of BMDMs from Hdac3f/f and Lyz2-Cre-Hdac3f/f mice upon LPS challenge, with biologic replicates, generated by RNA-seq technology.

2021-08-06 | GSE155801 | GEO

High-throughput sequencing Transcriptomes Analysis of WT and Kcnj2 KO mouse BMDMs treated with LPS or kir2.1 inhibitor

Project description:Purpose:Mouse BMDM is the universal cell type of studying innate immunity.This study was to analyze LPS induced innate immune response and the relationship between the inward rectifier potassium channel Kir2.1 and LPS induced innate immune respone Methods: WT and Kcnj2 KO BMDM cells were treated with or without LPS for 6 hours in the presence of kir2.1 inhibitor ML133 or not. Then mRNA profiles of these samles were generated by High-throughput sequencing analysis, in triplicate, using illumina HiSeq 2000. And the differential mRNA profiles were analyzed. Results: mRNA profiles of more than 20000 genes were analyzed, and differential expression profiles were compared . Conclusions: Our study represents the first detailed analysis of transcriptomes of WT and Kcnj2 KO mouse BMDM treated with LPS or kir2.1 inhibitor, with biologic replicates, generated by RNA-seq technology. This data was useful to analyze the relationship between Kir2.1 and LPS induced innate immune respone

2022-04-05 | GSE146158 | GEO

High-throughput RNA sequencing of six ant transcriptomes

Project description:High-throughput RNA sequencing of six ant transcriptomes

| PRJEB21038 | ENA

High-throughput sequencing analysis of mouse FABP5 KO BMDM cell transcriptomes with overexpression of FABP5 WT or FABP5 C127S after LPS treatment

Project description:Purpose: Mouse BMDM is the universal cell type of studying innate immunity. This study was designed to analyze LPS induced innate immune response and the gene expression in FABP5 KO BMDMs with overexpression of FABP5 WT or FABP5 C127S. Methods: FABP5 KO BMDMs were nucleofected with 5 μg pXJ40-3xFlag-FABP5 WT or C127S plasmid using the Amaxa Mouse Macrophage Nucleofector Kit (Lonza, VPA-1009) following the manufacturer’s instructions. Replace medium 6 hours post Nucleofection and add 500 ng/mL LPS to the fresh medium. 24 hours after treatment, harvest cells by using GenElute Single Cell RNA Purification Kit (Sigma, RNB300) and perform RNA-seq. Then mRNA profiles of these samples were generated by high-throughput sequencing analysis, using Illumina NovaSeq6000. And the differential mRNA profiles were analyzed. Results: mRNA profiles were analyzed, and differential expression profiles were compared. Conclusions: Our study demonstrated the transcriptional profiles of FABP5 KO BMDMs with overexpression of FABP5 WT or FABP5 C127S upon LPS treatment, with biologic replicates, generated by RNA-seq technology.

2021-11-01 | GSE182238 | GEO

High-throughput sequencing analysis of mouse FABP5 KO BMDM cell transcriptomes with overexpression of FABP5 WT or FABP5 C127S after LPS treatment

Project description:High-throughput sequencing analysis of mouse FABP5 KO BMDM cell transcriptomes with overexpression of FABP5 WT or FABP5 C127S after LPS treatment

| PRJNA755440 | ENA

High-throughput sequencing of small RNA transcriptomes in Locusta migratoria

Project description:All the reports on insect small RNAs come from holometabolous insects. However, small RNAs of hemimetabolous insects have not yet been investigated.Study of hemimetabolous insect small RNAs could provide more insights into evolution and function of small RNAs in hemi- and holometabolous insects. The locust is an important, economically harmful hemimetabolous insect and its phase changes is an interesting phenomenon.Here, we used high-throughput sequencing to characterize and compare the small RNA transcriptomes of gregarious and solitary phases in locusts. We found abundant small RNAs and their different expression profiles in the two phases. Small RNAs were sequenced from gregarious and solitary phases of Locusta migratoria,respectively.

2009-01-16 | E-GEOD-12640 | biostudies-arrayexpress

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