Project description:Pathogenic species belonging to Bacillus cereus sensu lato group possess a high evolutionary advantage in the environment and in food matrices thanks to their capacity to survive as silent spores to harsh environmental insults and grow at relatively low temperatures. Ready to re-heat products are at severe risk for contamination by members of Bacillus cereus s.l. group if not stored at proper conditions. In this work, the goal was to assess, by means of a genome-wide transcriptional assay, the isolated strain Bacillus cereus UC10070 gene expression behind the process of spore germination and consequent outgrowth in an artificially contaminated vegetable-based food model. A vegetable food model subjected to a heat treatment was determined to present favourable conditions for spores germination. Microscopic analyses together with OD measurements were applied to select the key steps of B. cereus cell cycle to be used for the microarray analysis. Using this approach we found a total of 1,646 probe sets differentially expressed and modulated during the entire B. cereus life cycle in the vegetable foodstuff. RNA was isolated from the dormant spores (DS), germinating spores at 40 minutes (GSP), early-log phase at 2h (C2h) and late log phase cells at 12 hours (C12h) after thermal treatment of B. cereus UC10070 previously isolated froma biofilm on a spoiled vegetable-based puree. There were 3 biological replicates (independent cultures) for each condition. Complete genome sequence of B. thuringiensis sv konkukian str. 97-27 (NCBI Reference Sequence: NC_005957.1) was chosen for its high homology with B. cereus s.l. UC10070, to design probes corresponding to 5,197 genes spotted in duplicates onto ElectraSenseH 12K microarrays chip.

2015-01-26 | E-GEOD-65259 | biostudies-arrayexpress

Bacillus sp. BB081

Project description:Bacillus cereus sensu lato BB081 Genome sequencing

| PRJNA475486 | ENA

Bacillus sp. JAS102

Project description:Bacillus cereus sensu lato JAS102 Genome sequencing

| PRJNA475494 | ENA

Temperature dependence of the proteome profile of the psychrotolerant pathogenic food spoiler Bacillus weihenstephanensis type strain WSBC 10204

Project description:Bacillus weihenstephanensis is a subspecies of the Bacillus cereus sensu lato group of spore forming bacteria known to cause food spoilage or food poisoning. The key distinguishing phenotype of B. weihenstephanensis is its ability to grow below 7°C or, from a food safety perspective, to grow and potentially produce toxins in a refrigerated environment. In order to gain insight into to the mechanistic basis of its psychrotolerant phenotype, as well as elucidate relevant aspects of its toxigenic profile, the proteome profiles of cells grown at either 6°C or 30°C were compared.

2015-12-01 | PXD002007 | Pride

The effect of the pBCXO1 plasmid on the transcriptome of Bacillus cereus G9241 at both 37 and 25 degree celsius

Project description:RNA-seq experiment comparing the transcriptomes of Bacillus cereus G9241 WT to B. cereus G9241 ∆pBCXO1 when cultured both 37 and 25 degree celsius. B. cereus G9241 is a B. cereus sensu stricto strain that was isolated from a welder with and anthrax-like illness. B. cereus G9241 carries the plasmids pBCXO1 and pBC210. pBCX01 has 99.6% sequence identity to pXO1 carried by Bacillus anthracis and encodes the tripartite anthrax toxin genes and atxA, a mammalian virulence transcriptional regulator. B. cereus G9241 WT and B. cereus G9241 ∆pBCXO1 were cultured to exponential phase at either 37 or 25 degree celsius before samples were taken for RNA extraction, library prep and sequencing.

2023-05-13 | E-MTAB-12883 | biostudies-arrayexpress

Bacillus sp. AR18-7

Project description:Bacillus cereus sensu lato AR18-7 Genome sequencing

| PRJNA475491 | ENA

Bacillus sp. BF9-10

Project description:Bacillus cereus sensu lato BF9-10 Genome sequencing

| PRJNA475492 | ENA

Bacillus sp. AY1-10

Project description:Bacillus cereus sensu lato AY1-10 Genome sequencing

| PRJNA475493 | ENA