Project description:Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a respiratory disease which causes great economic losses worldwide. Many virulence factors are involved in the pathogenesis, namely capsular polysaccharides, RTX toxins, LPS and many iron acquisition systems. In order to identify genes that are expressed in vivo during a natural infection, we undertook transcript profiling experiments with an A. pleuropneumoniae DNA microarray, after recovery of bacterial mRNAs from serotype 5b-infected porcine lungs. Comparative Genomic Hybridizations between Actinobacillus pleuropneumoniae serotype 5b strain L20 (ref) and serotype 5b fresh field isolate 896-07, recovered from infected pig lung tissues following natural acute infection. Two condition transcript profiling experiments : infectious 5b field strain isolated directly from lungs of naturally deceased pigs after acute infection vs infectious 5b field strain grown in BHI broth to an OD600 of 0.300.

2010-05-05 | E-GEOD-15911 | biostudies-arrayexpress

Comparative transcriptome analysis reveals metabolic differences between planktonic and biofilm of Actinobacillus pleuropneumoniae.

Project description:To reveal the transcriptional profiles of Actinobacillus pleuropneumoniae under biofilm and planktonic growth, we established a biofilm-forming culture method and constructed a mutant strain Δpga with defect in biofilm formation. Wild-type and Δpga mutant strains of Actinobacillus pleuropneumoniae strain 4074 were cultured in bottles with shaking for planktonic (WT_PK) and in microplates in static status for biofilm (WT_BF, Δpga), respectively. The bacteria in logarithmic growth period of different culture groups were collected for RNA seq.

2023-05-31 | GSE221940 | GEO

Actinobacillus pleuropneumoniae strain:1140

Project description:Actinobacillus pleuropneumoniae strain:1140 Genome sequencing and assembly

| PRJNA684043 | ENA

Actinobacillus pleuropneumoniae strain:1022

Project description:Actinobacillus pleuropneumoniae strain:1022 Genome sequencing and assembly

| PRJNA266228 | ENA

Actinobacillus succinogenes strain:GXAS137

Project description:Actinobacillus succinogenes strain:GXAS137 Genome sequencing and assembly

| PRJNA386711 | ENA