Project description:To investigate the role of gene expression during Newcastle disease virus (NDV) infection.The NDV GM strain was used to infect DEF cells with 1moi, while an uninfected group was set up as a control.
Project description:We report the genetic plasticity of Newcastle disease virus. We introduced insertional mutation in the virus genome and checked fitness by comparing distribution of mutants in passage 1 and passage 2.
Project description:Newcastle disease (ND) is an acute, febrile, and highly contagious disease caused by the virulent Newcastle disease virus (vNDV) worldwide. The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of vNDV. DF-1 cells and the lungs of specific pathogen free (SPF) chickens infected with the vNDV strain Herts/33 were analyzed via ultra high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 304 metabolites were significantly changed post vNDV infection, and most belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may be used for viral protein synthesis and genome amplification. Similar results were also confirmed in vivo; this is consistent with the characteristic of acute vNDV infection. The identification of these different metabolites will provide a great deal of important information to further understand the mechanism of vNDV replication and pathogenesis.
Project description:This study aimed to characterize differential responses between the relatively resistant (Fayoumi) and susceptible (Leghorn) chicken lines when challenged with a high titered lentogenic Newcastle Disease Virus (NDV) strain via the eyes and nostrils at three weeks of age. The trachea epithelial cells were collected from challenged and non-challenged birds from each line at 2, 6, and 10 days-post-infection (dpi). High quality RNA was isolated, used for cDNA library construction, and sequenced on the HiSeq2500.
Project description:This study aimed to characterize differential responses between the relatively resistant (Fayoumi) and susceptible (Leghorn) chicken lines when challenged with a high titered lentogenic Newcastle Disease Virus (NDV) strain via the eyes and nostrils at three weeks of age. The lung was collected from challenged and non-challenged birds from each line at 2, 6, and 10 days-post-infection (dpi). High quality RNA was isolated, used for cDNA library construction, and sequenced on the HiSeq2500.
Project description:Newcastle disease virus (NDV) is an avian paramyxovirus that causes major economic losses to the poultry industry around the world, with NDV pathogenicity varying due to strain virulence differences. However, the impact of intracellular viral replication and the heterogeneity of host responses among cell types are unknown. Here, we investigated the heterogeneity of lung tissue cells in response to NDV infection in vivo and that of chicken embryo fibroblast cell line DF-1 to NDV infection in vitro using single-cell RNA sequencing.
Project description:Airway epithelial cells from 3 different breeds of chicken infected with Newcastle Disease virus were sequenced and compared to cells from uninfected control birds. The 3 breeds were an indigenous breed, commercial Rhode Island Reds and a hybrid breed (Kenbro).
