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Comparative transcriptome profiling of Chinese tongue sole in immune response against Vibrio harveyi infection

ABSTRACT: Comparative transcriptome profiling of Chinese tongue sole in immune response against Vibrio harveyi infection

PROVIDER: PRJNA524115 | ENA |

REPOSITORIES: ENA

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Comparative transcriptome profiling of Chinese tongue sole in immune response against Vibrio harveyi infection

Project description:Vibrio harveyi is a major bacterial pathogen that can cause fatal vibriosis in Chinese tongue sole (Cynoglossus semilaevis). To comprehend the molecular mechanisms of C. semilaevis host response against V. harveyi infection, we performed transcriptome (RNA-seq) analysis of C. semilaevis from resistant family and susceptible family.

2019-02-26 | GSE126995 | GEO

Comparative analysis of male and pseudomale gonads in Chinese tongue sole

Project description:Comparative analysis of male and pseudomale gonads in Chinese tongue sole

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Transcriptome data involved in female Chinese tongue sole (Cynoglossus semilaevis)

Project description:Transcriptome data involved in female Chinese tongue sole (Cynoglossus semilaevis)

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Comparative transcriptome profiling of immune response against Vibrio harveyi infection in Chinese tongue sole.

Project description: Not available

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Transcriptome data involved in Chinese tongue sole (Cynoglossus semilaevis) sexual growth dimorphism

Project description:Transcriptome data involved in Chinese tongue sole (Cynoglossus semilaevis) sexual growth dimorphism

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Identify and characterize the circular RNAs expressed in in the half-smooth tongue sole (Cynoglossus semilaevis)

Project description:Half-smooth tongue sole (Cynoglossus semilaevis) is a commercially valuable flatfish, ranking among the most valuable fishes in the coastal areas of China. It has an asymmetric body shape with lateralization of the eyes to the same side during metamorphosis as same as the other flatfish species. However, little is known about the function and profile of circRNAs in the half-smooth tongue sole. In this study,a total of 2730 circRNAs were identified from in the fish by deep RNA-sequencing technique.

2017-09-27 | GSE103984 | GEO

Genome-wide identification and prediction of long non-coding RNAs in the half-smooth tongue sole (Cynoglossus semilaevis)

Project description:Half-smooth tongue sole (Cynoglossus semilaevis) is a commercially valuable flatfish, ranking among the most valuable fishes in the coastal areas of China. It has an asymmetric body shape with lateralization of the eyes to the same side during metamorphosis as same as the other flatfish species. However, little is known about the function and profile of lncRNAs in the half-smooth tongue sole. In this study, we carry out a genome-wide review of lncRNAs in the fish by using large-scale deep sequencing.

2017-09-27 | GSE98425 | GEO

Gonadal DNA methylomes of half-smooth tongue sole Cynoglossus semilaevis

2014-01-23 | GSE41129 | GEO

Gonadal DNA methylomes of half-smooth tongue sole Cynoglossus semilaevis

Project description:Environmental sex determination (ESD) occurs in divergent, phylogenetically unrelated taxa, and in some species co-occurs with genetic sex determination (GSD) mechanisms. Although epigenetic regulation in response to environmental effects has long been proposed to be associated with ESD, a systemic analysis on epigenetic regulation of ESD is still lacking. Using half-smooth tongue sole (Cynoglossus semilaevis) as a model – a marine fish which has both ZW chromosomal GSD and temperature-dependent ESD – we investigated the role of DNA methylation in transition from GSD to ESD by comparing gonadal DNA methylomes of parental females, parental pseudo-males, F1 females, F1 pseudo-males and normal males. To assess the gonadal DNA methylome patterns across different sexual types of tongue sole, we carried out BS-seq on bisulfite converted DNA extracted from adult gonads of parental females, parental pseudo-males, and F1 pseudo-males and females from a cross between a parental pseudo-male and a normal female. We also sampled normal male individuals as a control for the normal male DNA methylation pattern. For each of the five samples, two biological replicates were utilized, with each replicate being pooled by five fish. The phenotype and genotype of each selected fish was identified by the histological analysis and PCR validation using the W chromosome specific marker. DNA were isolated from five pooled gonads of the same replicate, then 5 ?g DNA was used to do the bisulfite conversion and BS-seq. The bisulfite conversion of sample DNA was carried out using a modified NH4HSO3-based protocol (Hayatsu et al. 2006). The paired-end library construction and sequencing were carried out using Illumina HiSeq 2000, according to the manufacturer’s instructions (Illumina). We also mixed 25 ng cl857 Sam7 Lambda DNA in each sample to use as conversion quality control for each library.

2014-01-23 | E-GEOD-41129 | biostudies-arrayexpress

Global profiling of megalocytivirus-induced proteins in tongue sole (Cynoglossus semilaevis) spleen identifies cellular processes essential to viral infection

Project description:In this study, label-free proteomics was performed to examine protein expression in tongue sole spleen induced by megalocytivirus at 8 and 12 days post infection (dpi)

2018-10-25 | PXD011479 | iProX

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