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Gene expression analysis of human CD34+ AML cells cultured with or without gabapentin

ABSTRACT: Gene expression analysis of human CD34+ AML cells cultured with or without gabapentin

PROVIDER: PRJNA524297 | ENA |

REPOSITORIES: ENA

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Gene expression analysis of human CD34+ AML cells cultured with or without gabapentin

Project description:To clarify the global gene expression alteration in primary CD34+ AML cells after BCAA metabolism inhibition, CD34+ AML cells were treated with or without gabapentin in vitro. After 9-12 hours of culture with or without gabapentin, FACS-purified CD34+ AML cells were subjected to transcriptome analysis.

2019-06-01 | GSE127181 | GEO

Gene expression analysis of human CD34+ ALL cells cultured with or without gabapentin

Project description:To clarify the global gene expression alteration in primary CD34+ ALL cells after BCAA metabolism inhibition, CD34+ AML cells were treated with or without gabapentin in vitro. After 9-12 hours of culture with or without gabapentin, FACS-purified CD34+ ALL cells were subjected to transcriptome analysis.

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Gene expression analysis of human CD34+ ALL cells cultured with or without gabapentin

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Transcription profiling of human CD34+ fractions from AML for chromosome copy number analysis

Project description:CD34-positive fractions were used for mRNA preparation, which was hybridized to HGU 133 Plus 2.0 arrays. Experiment Overall Design: CD34-positive fractions of 22 AML patients were analyzed.

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Gene expression profiling in the leukemic stem cell-enriched CD34+ fraction identifies target genes that predict prognosis in normal karyotype AML

Project description:Mononuclear cells from AML patients (n=46) were sorted into CD34+ and CD34- subfractions and genome-wide expression analysis was performed using Illumina BeadChip Arrays (HT12 v3). Of 2 AML samples only the CD34+ fraction could be analyzed. AML CD34+ and CD34- gene expression was compared to a large group of normal CD34+ bone marrow cells (n=31). Mononuclear cells from AML patients (n=46) were sorted into CD34+ (46) and CD34- (44) subfractions and genome-wide expression analysis was performed using Illumina BeadChip Arrays (HT12 v3). AML CD34+ and CD34- gene expression was compared to a large group of normal CD34+ bone marrow cells (n=31).

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Gene expression change induced by TIM-3/galectin-9 interaction in primary AML

Project description:Analysis of gene expression change after galectin-9 stimulation in primary CD34+TIM-3+ AML cells Total RNA obtained from purified CD34+TIM-3+ primary AML cells with or without recombinant human galectin-9 stimulation (20hrs) was subjected to transcriptome analysis

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