Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of clinical breast cancer cells cultured by sphere and adherent culture methods

ABSTRACT: Gene expression of clinical breast cancer cells cultured by sphere and adherent culture methods

PROVIDER: PRJNA524635 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Human colorectal cancer cell line CRC#21: Sphere-cultured cells vs. Adherent-cultured cells

Project description:Transcriptional profiling of cancer stem cells (sphere-cultured cells) comparing non-cancer stem cells (adherent-cultured cells). Goal was to identity cancer stem cell-specific genes. Two-condition experiment, sphere-cultured cells vs. adherent-cultured cells: 1 sphere-cultured replicate and 1 adherent-culture replicate.

2016-04-01 | E-GEOD-77150 | biostudies-arrayexpress

Human colorectal cancer cell line CRC#21: Sphere-cultured cells vs. Adherent-cultured cells

2016-04-01 | GSE77150 | GEO

CaSki cells: adherent cultured cell vs. sphere cells

Project description:Transcriptional profiling of human cervical squamous cell carcinoma cell line CaSki comparing adherent cultured cells with spheres cultured in serum free culture medium. Goal was to identify candidate antigens for immunotherapy targeting cancer stem cells. Two-condition experiment, CaSki vs. CaSki-sphere cells.

2015-03-04 | E-GEOD-65499 | biostudies-arrayexpress

Gene expression of clinical breast cancer cells cultured by sphere and adherent culture methods

Project description:Cancer stem-like cells (CSCs) are thought to be responsible for recurrence of tumors and poor prognosis because of their higher tumor initiating ability and drug resistance, though the mechanisms remain still unclear. Here, we show a critical role of mini-chromosome maintenance protein (MCM) 10, a component of DNA replication machinery, in enabling CSCs to deal with DNA replicative stress. Our transcriptomic analysis of patient-derived cultured breast cancer cells revealed that MCM10 is strongly upregulated in CSC-enriched spheroid cells, compared to cancer cells cultured in a regular condition. Elevated MCM10 expression was correlated with worse prognosis of patients with breast cancer. Depletion of MCM10 resulted in not only reduced cell proliferation and tumorigenesis, but also reduced tumor sphere forming efficiency, expression levels of stemness markers, and the tumor initiating ability. These results indicate that MCM10 is essential for maintenance of CSCs as well as for tumor cell proliferation. DNA fiber assay revealed that CSCs have a comparatively high level of DNA replicative stress. Overexpression of MCM10 helped cells to proliferate in a medium containing hydroxyurea (HU), a replicative stress-inducing chemotherapeutic agent. Conversely, MCM10 depletion made cells more fragile to replicative stress induced by HU or mitomycin C (MMC), another replicative stress inducer. Therefore, MCM10 plays critical roles for dealing with the enhanced replicative stress generated in CSCs for their survival, leading to maintenance of tumor initiating ability and drug resistance. We provide a preclinical rationale to target MCM10, the critical mediator for DNA replicative stress, for breast cancer that includes CSCs.

2021-09-09 | GSE127264 | GEO

Chondrosarcoma cells: sphere vs adherent

Project description:Transcriptional profiling of human chondrosarcoma sphere vs adherent cells

2015-04-30 | GSE47823 | GEO

Chondrosarcoma cells: sphere vs adherent

Project description:Transcriptional profiling of human chondrosarcoma sphere vs adherent cells Two different growth conditions, sphere vs adherent

2015-04-30 | E-GEOD-47823 | biostudies-arrayexpress

CaSki cells: adherent cultured cell vs. sphere cells

2015-03-04 | GSE65499 | GEO

mRNA-seq of mouse salivary gland cancer cells cultured in adherent or non-adherent culture

Project description:Aim: investigate how the Wnt-driven Mll1 epigenome regulates salivary gland and head and neck cancer. We performed mRNA-seq and ChIP-seq of H3K4me1, me2 and me3 on mouse salivary gland cancer cells that are kept in two different growth conditions, adherent culture and non-adherent sphere culture. Mouse salivary gland cancer cells were isolated from salivary gland of transgenic mouse that harbor K14-Cre-induced Wnt/β-catenin gain-of-function and Bmpr1a loss-of-function mutations.

2018-12-24 | E-MTAB-7509 | biostudies-arrayexpress

Expression data from glioblastoma derived sphere lines, adherent cell lines and their original glioblastoma tumor

Project description:Glioblastoma (GBM) derived sphere lines and adherent cell lines are an important tool for research in basic and translational neuro-oncology. Documentation of their genetic identity has become a requirement for scientific journals and grant applications to exclude cross-contamination and misidentification that lead to misinterpretation of results. Here, we report expression data for 26 samples including 4 GBM derived sphere lines (4 x 3 replicates), 2 GBM derived sphere lines passaged through intracranial transplantation (2x 1), 2 adherent GBM derived cell lines (2 + 2 x 3 replicates), 4 corresponding glioblastoma tumors and 2 non-tumor brain tissues.

2017-09-27 | GSE104291 | GEO

Expression data of 3 prostate cancer stem cell primary lines comparing spheres and parental/adherent culture

Project description:Transcriptional profile of PCSC spheres in SCM-1% KO (stem-like cells) vs adherent cultures in PCSC-Celprogen medium (differentiated-like cells) Two-condition experiment: Sphere vs. Parental/adherent cells. Biological replicates: 2 sphere replicates , 2 adherent replicates.

2010-01-01 | GSE19713 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data