Project description:The maize B chromosome is a non-essential chromosome and is not detrimental to plant development at low copy numbers. Nondisjunction of the maize B chromosome occurs at the second pollen mitosis. Maize lines containing B chromosomes are widely used in a variety of studies, such as gene dosage effect, centromere function and engineering of artificial chromosomes etc. An understanding of how the B chromosome affect gene expression is required for utilizing the B chromosome as tools for genetic studies and engineering. Therefore, we performed RNA-seq and small-RNA seq experiments on maize lines containing one through seven copies of B chromosomes to examine how gene expression responds to change of B copy numbers.
Project description:This was a pilot project carried out by Dr Wojciech Majeran to determine the maize pollen proteome harvested from field-grown W22 (T43) plants in plots on the Musgrave Research Farm (Cornell CALS) in Aurora (NY). To enhance proteome coverage, the pollen were separated into soluble and membrane bound protein fractions, and separated by SDS-PAGE followed by in-gel digestion and shot-gun proteomics using a nanoLC-Orbitrap system.
Project description:Large scale transcriptomics study to establish gene expression in leaf tissue of W22 inbred line in Zea Mays. RNA was extracted from leaf tissue when the plants were at V6. Sequencing library was produced following the protocol mentioned in the following publication PMID:22039485
Project description:sRNA-seq profiling of 10 time points during germination in Arabidopsis, from freshly harvested seed, through mature seed, stratification, germination and to post-germination.