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LncRNA expression profiles in spermatocyte cell line (GC2) after exposure to triptolide and triptonide

ABSTRACT: lncRNA expression profiles in spermatocyte cell line (GC2) after exposure to triptolide and triptonide

PROVIDER: PRJNA525002 | ENA |

REPOSITORIES: ENA

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lncRNA expression profiles in spermatocyte cell line (GC2) after exposure to triptolide and triptonide

Project description:Triptolide or triptonide, major bioactive components isolated from the traditional Chinese herb Tripterygium wilfordii Hook f. (TWHF), have been shown to exert various pharmacological effects on systemic autoimmune disease. However, the severe toxicity, especially reproduction toxicity, of triptolide or triptonide prevents wide clinical use for those people with fertility needs. Long noncoding (lnc) RNAs are critical for spermatogenesis, and their dysregulation might lead to male infertility. However, whether they are involved in triptolide or triptonide -induced reproduction toxicity is unknown. Here we found that exposure of spermatocyte cell line (GC2) to triptolide or triptonide led to reduction in cell viability and induced cell apoptosis and autophagy. Then the total RNAs of triptolide or triptonide -treated GC2 cells were used to investigate lncRNA expression profiles by strand-specific RNA sequencing at the transcriptome level to help determine any RNA-related mechanisms in triptolide or triptonide-induced toxicity. Gene ontology and pathway analyses showed that the functions of differentially expressed lncRNA targets and mRNAs were closely linked with many processes involved in spermatogenesis.

2022-03-01 | GSE127662 | GEO

Dysregulation of lncRNAs and circRNAs in mouse testes after exposure to triptolide

Project description:Triptolide has been shown to exert various pharmacological effects on systemic autoimmune diseases and cancers. However, its severe toxicity, especially reproduction toxicity, prevents wide clinical use for those people with fertility needs. Noncoding RNAs including lncRNAs and cicrRNAs are regulatory molecules that mediate a wide variety of physiological activities, which are critical for spermatogenesis, and their dysregulation might lead to male infertility. However, whether they are involved in triptolide-induced reproduction toxicity is fully unknown. Here, after exposure of mice to triptolide for 35 day, the total RNAs were used to investigate lncRNAs/cicrRNAs/mRNAs expression profiles by strand-specific RNA sequencing at the transcriptome level to help determine any RNA-related mechanisms in triptolide-induced toxicity. The results showed that triptolide significantly decreased the testicular weight, damaged testis and sperm morphology, reduced sperm motility and density. Furthermore, there's a remarkable deformity in sperm head and tail in triptolide-exposed mice. At the transcriptome level, the triptolide-treated mice exhibited aberrant expression profiles for lncRNAs/cicrRNAs/mRNAs, many of them were dysregulated. Gene ontology and pathway analyses revealed that the functions of differentially expressed lncRNA targets, cicrRNAs cognate genes and mRNAs were closely linked with many processes involved in spermatogenesis. Additionally, many newly identified lncRNAs /cicrRNAs displayed inducible expression, suggesting that they might be good candidate markers for triptolide-induced male reproductive toxicity. This study gives us an insight into understanding the reproductive system toxicity of triptolide and reminds us to strengthen the research on increasing efficacy and decreasing toxicity of triptolide.

2020-03-23 | GSE126857 | GEO

Analyses of mIRNA transcript level following exposure to triptolide

Project description:Analyses of mirna transcript levels following exposure to the drug triptolide in vitro and prodrug minnelide in xenograft pancreatic tumor mouse models. Response of pancreatic adenocarcinoma cell lines to minelide in vitro and exposure to its prodrug triptolide in SCID mouse tumors derived from the same cell lines.

2013-11-12 | E-GEOD-46188 | biostudies-arrayexpress

Analyses of mIRNA transcript level following exposure to triptolide

Project description:Analyses of mirna transcript levels following exposure to the drug triptolide in vitro and prodrug minnelide in xenograft pancreatic tumor mouse models.

2013-11-12 | GSE46188 | GEO

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [miRA-seq]

Project description:Zebrafish larvae treated with triptolide (5 dpf larvae after exposure to triptolide (1.6μM) for 6 hours) or vehicle control.

2020-03-03 | GSE146200 | GEO

Characterization of Triptolide-Induced Hepatotoxicity by Imaging and Transcriptomics in a Novel Zebrafish Model [RNA-seq]

Project description:Zebrafish larvae treated with triptolide (5 dpf larvae after exposure to triptolide (1.6μM) for 6 hours) or vehicle control.

2020-03-03 | GSE146199 | GEO

Triptolide alters the miRNA expression profiles in human hepatocellular carcinoma HepG2 cells

Project description:We compared miRNA expression profiles among 5 groups of HepG2 cells treated with various concentrations (0,100,200 nM) of triptolide for 12 h or 24 h. Two-condition experiment, triptolide intervention vs. without intervention. Biological replicates: 3 control, 3 treated, independently grown and harvested. One replicate per array.

2014-05-01 | E-GEOD-40037 | biostudies-arrayexpress

Triptolide alters the miRNA expression profiles in human hepatocellular carcinoma HepG2 cells

Project description:We compared miRNA expression profiles among 5 groups of HepG2 cells treated with various concentrations (0,100,200 nM) of triptolide for 12 h or 24 h.

2014-05-01 | GSE40037 | GEO

Dysregulation of lncRNAs and circRNAs in mouse testes after exposure to triptolide

Project description:Dysregulation of lncRNAs and circRNAs in mouse testes after exposure to triptolide

| PRJNA523537 | ENA

Expression data from treatment of actinomycin D and triptolide on MCF7 cells

Project description:Expression data from treatment of actinomycin D (2.5uM) and triptolide (500 nM) on MCF7 cells for 2, 4 and 6 hours. We used microarrays to explore the mechanism of triptolide action.

2011-10-18 | GSE28662 | GEO

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