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Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes [4sURDB-Seq]

ABSTRACT: Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes [4sURDB-Seq]

PROVIDER: PRJNA525151 | ENA |

REPOSITORIES: ENA

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Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes

Project description:Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes

| PRJNA525152 | ENA

Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes [RNA-seq]

Project description:Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes [RNA-seq]

| PRJNA516337 | ENA

Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes [4sURDB-Seq]

Project description:The rate of RNA Polymerase II (RNAPII) elongation has an important role in the control of Alternative splicing (AS); however, the in vivo consequences of an altered elongation rate are unknown. Here, we generated mouse embryonic stem cells (ESCs) knocked-in for a slow elongating form of RNAPII. We show that a reduced transcriptional elongation rate results in early embryonic lethality in mice and impairs the differentiation of ESCs into the neural lineage. This is accompanied by changes in splicing and in gene expression in ESCs and along the pathway of neuronal differentiation. In particular, we found a crucial role for RNAPII elongation rate in transcription and splicing of long neuronal genes involved in synapse signaling. The impact of the kinetic coupling of RNAPII elongation rate with AS is more predominant in ESC-differentiated neurons than in pluripotent cells. Our results demonstrate the requirement for an appropriate transcriptional elongation rate to ensure proper gene expression and to regulate AS during development.

2019-04-15 | GSE127740 | GEO

Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes [RNA-seq]

2019-04-15 | GSE125413 | GEO

A slow transcription rate causes embryonic lethality and perturbs kinetic coupling of neuronal genes.

Project description: Not available

| S-EPMC6484407 | biostudies-literature

Slow transcriptional elongation causes embryonic lethality and perturbs kinetic coupling of long neural genes

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-04-15 | GSE127741 | GEO

Synthetic lethality/slow growth with acs2-Ts8

Project description:Obtain synthetic lethality/slow growth gene interactors of acs2-Ts8 Keywords: gene mutation

2006-10-01 | GSE4144 | GEO

A slow transcription rate causes embryonic lethality and perturbs kinetic coupling of neuronal genes

Project description: Not available

| S-SCDT-EMBOJ-2018-101244 | biostudies-other

Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast - 4tU cordycepin treatment with rpb1 slow mutant

Project description:mPAT approach using an anchored oligo-dT (TV12VN) primer to determine changes to 3'UTR length in yeast with a slow RNA polymerase II mutant rpb1-1, complemented with wild-type RPB1 or a slow rpb1 mutant plasmid, upon cordycepin treatment and new transcription using 4-thiouracil (4tU) labelling of RNA. This appears as a T to C conversion in sequencing reads.

2021-06-25 | GSE160994 | GEO

SML1 synthetic lethality

Project description:Assessment of synthetic lethality between an sml1 null mutation and Yeast Deletion mutations Keywords = SML1 Keywords = yeast Keywords = deletion Keywords = synthetic lethality Keywords = tag Keywords = barcode Keywords: repeat sample

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