Project description:The ability of Cupriavidus oxalaticus T2 to simultaneously remove nitrogen and phenol has been confirmed. To explore the metabolic characteristics and adaptive mechanism of Cupriavidus oxalaticus T2 during the simultaneous removal of phenol and nitrogen process, the differences in proteomic profile after supplement with phenol and ammonia for 6 h (lag phase) and 24 h (log phase) were evaluated. The results revealed that a new potential phenol para-degradation pathway appeared in T2. Phenol induces changes in nitrogen metabolism resulting in increased denitrification and decreased synthesis of glutamate from ammonia at 6 h. In addition, phenol exposure enhanced the expression of cytochrome oxidases with high oxygen affinity and increased ATP synthesis. The increase of chemotaxis and flagellar assembly was conducive to the uptake and utilization of phenol. The synthesis of lipoic acid and biotin was promoted to resistance phenol toxicity. Moreover, phenol triggered cellular stress response thereby leading to th
