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Transcriptome analysis of erythroblastic island macrophages

ABSTRACT: Transcriptome analysis of erythroblastic island macrophages

PROVIDER: PRJNA525977 | ENA |

REPOSITORIES: ENA

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Transcriptome analysis of erythroblastic island macrophages

Project description:The erythroblastic island (EBI), composed of a central macrophage and surrounding erythroid cells, was the first hematopoietic niche discovered. The identity of EBI macrophages has thus far remained elusive. Gene expression profiles of BM F4/80+Epor-eGFP+ macrophages suggest a specialized function in supporting erythropoiesis.

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PPARγ is essential for the development of erythroblastic island macrophages and red pulp macrophages

Project description:Macrophages in the bone marrow erythroblastic island (EIM) and splenic red pulp (RPM) are required for terminal erythropoiesis and removal of aged erythrocytes, respectively. This manuscript shows that EIM and RPM development require both PPARg and Spi-C.

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PPARγ is essential for the development of erythroblastic island macrophages and red pulp macrophages

Project description:PPARγ is essential for the development of erythroblastic island macrophages and red pulp macrophages

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Transcriptome wide analysis of classically and alternatively activated macrophages

Project description:Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived human M1- and M2-like macrophage profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to establish a high resolution transcriptome of human macrophages. Total RNA was isolated from classically and aternative activated human macrophages.mRNA profiles were generated by deep sequencing og M1 and M2 macrophages from 3 donors using Illumina HiSeqSQ. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Casava1.8 and TopHat followed by Cufflinks. qRT–PCR validation was performed using LightCycler and SYBR Green assays. mRNA profiles were generated by deep sequencing og M1 and M2 macrophages from 3 donors using Illumina HiSeqSQ.

2012-10-01 | E-GEOD-36952 | biostudies-arrayexpress

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Project description:RNA-seq analysis of cardiac, renal, and liver macrophages. CD11b+F4/80+Ly6C-Ly6G- tissue resident macrophages were isolated from the heart, kidney, and liver of mice. Isolated RNA was subjected to RNA-seq to identify differentially expressed transcripts.

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Methylation detection Oligonucleotide Microarray Analysis: high resolution method for CpG island methylation detection

Project description:This SuperSeries is composed of the following subset Series: GSE15746: Methylation detection Oligonucleotide Microarray Analysis: high resolution method for CpG island methylation detection 1 GSE15747: Methylation detection Oligonucleotide Microarray Analysis: high resolution method for CpG island methylation detection 2 Refer to individual Series

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Analysis of lncRNAs in macrophages

Project description:Analysis of lncRNAs in macrophages

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Transcriptome analysis of alveolar macrophages in asthma

Project description:Purpose: The goal of this study is to investigate the alteration of gene expression pattern of alveolar macrophages by allergen challenge in human asthmatics. Method: By using subsegmental bronchial provocation with allergen (SBP-AG) protocol, we obtained BAL fluids, before and 48 hours after allergen challenge in the subjects enrolled in the protocol. Alveolar macrophages were purified from the BAL fluids and total RNA was isolated. Next-generation sequencing data were generated by using the Illumina system. Results: Using an optimized data analysis workflow, we mapped about 75 million sequence reads per sample to the human genome and identified 29,691 transcripts in the macrophage mRNAs. Among them, the change in the expression profiles of 37 transcripts were statistically significant. Conclusions: It has been well accepted that Th2 cytokine enriched environment transforms the phenotype of macrophages into alternatively activated form. However, the details of a genome-wide gene expression profiles of macrophages were not well investigated. Using RNA-seq technology, we provided comprehensive data of macrophage gene expression profiles in allergic lung inflammation. Our data could offer a framework to study biologic functions of alternatively activated macrophage in chronic inflammatory diseases. mRNA profiles of alveolar macrophages obtained from asthmatics, before and after allergen challenge.

2015-04-01 | E-GEOD-65039 | biostudies-arrayexpress

Transcriptome wide analysis of untreated, classically and alternatively activated macrophages

Project description:Analysis of human M0-, M1-, and M2-like macrophages. Total RNA was isolated from untreated, classically and aternative activated human macrophages

2012-10-01 | E-GEOD-35449 | biostudies-arrayexpress