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Eomes and Brachyury control pluripotency exit and germ-layer segregation by changing the chromatin state

ABSTRACT: Eomes and Brachyury control pluripotency exit and germ-layer segregation by changing the chromatin state

PROVIDER: PRJNA527809 | ENA |

REPOSITORIES: ENA

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Eomes and Brachyury control pluripotency exit and germ-layer segregation by changing the chromatin state

Project description:We aimed to elucidate molecular mechanisms of first lineage decision in the mouse pluripotent epiblast that segregates mesoderm and endoderm (ME) from neuroectoderm (NE). By analyzing mouse embryonic stem cells (ESCs) and embryos deficient for two T-box (Tbx) transcription factors Eomes and Brachyury we demonstrate that this process is controlled by the changes in the chromatin accessibility of ME gene enhancers and activation of the target genes, but also direct repression the opposing pluripotency and NE gene programs.

2019-12-02 | GSE128466 | GEO

The molecular basis of specific functions of Brachyury and Eomes for mesoderm and endoderm lineage specification

Project description:The Tbx factors Eomesodermin (Eomes) and Brachyury instruct endoderm and mesoderm specification. Both Tbx factors have common large overlap in chromatin binding sites, however their embryonic phenotypes of mutants largely differ. In this study, we delineate the distinct binding patterns and gene target sets of Eomes and Brachyury providing a molecular model of distinct fate specification programs.

2023-07-26 | GSE194192 | GEO

Zebrafish brachyury chipseq

Project description:ChIP-seq with antibodies against Brachyury (Ntl), Tbx16 (Spt) and Eomes. 3 experiments 2 samples each (one lane per sample). This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2011-11-10 | E-ERAD-28 | biostudies-arrayexpress

Tbet-Eomes

Project description:Tbet-Eomes

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Gene expression of sorted Eomes+ and Eomes‒ γδ T cells from spleen of Eomes-IRES-GFP mice.

Project description:Eomesodermin (Eomes) is a transcription factor with a crucial role regulating cytotoxic function, development and survival of immune cells. Although it is known that γδ T cells can express Eomes, its function on those cells is still largely unknown. Using Eomes-IRES-GFP mice we were able to sort for Eomes+ and Eomes‒ γδ T cells populations and get their gene expression profiles, bringing light to the role of Eomes on γδ T cells.

2017-04-10 | GSE85585 | GEO

anti-Eomes ChIPseq of control OT-I cells and Eomes-overexpressing OT-I cells

Project description:High amount of Eomes might drive T cell exhaustion. In order to understand how Eomes contributes to exhaustion of CD8+ T cell in the TME as a transcription factor, we conducted anti-Eomes ChIPseq analysis of control OT-I cells and Eomes-overexpressing OT-I cells.

2018-11-26 | GSE122888 | GEO

EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming

Project description:Memory CD8+ T cells have the ability to provide lifelong immunity against pathogens. Although memory features generally arise after challenge with a foreign antigen, naïve CD8 single positive (SP) thymocytes may acquire phenotypic and functional characteristics of memory cells in response to cytokines such as interleukin-4. This process is associated with the induction of the T-box transcription factor Eomesodermin (EOMES). However, the underlying molecular mechanisms remain ill-defined. Using epigenomic profiling, we show that these innate memory CD8SP cells acquire only a portion of the active enhancer repertoire of conventional memory cells. This reprograming is secondary to EOMES recruitment, mostly to RUNX3-bound enhancers. Furthermore, EOMES is found within chromatin-associated complexes containing BRG1 and promotes the recruitment of this chromatin remodelling factor. Also, the in vivo acquisition of EOMES-dependent program is BRG1-dependent. In conclusion, our results support a strong epigenetic basis for the EOMES-driven establishment of CD8+ T cell innate memory program.

2019-06-06 | PXD014142 | Pride

Probing Brachyury Function during ES cell differentiaiton

Project description:To probe how Brachyury regulates its target genes, we performed mRNA-seq to analyze gene expression changes after Brachyury depletion by the lentivirus-mediated shRNA

2014-03-10 | GSE54984 | GEO

Map Brachyury-bound enhancers by ChIP-seq

Project description:To gain new mechanistic insights into highly-regulated lineage specification and morphogenetic processes during early embryogenesis, we applied ChIP-seq to identify transcriptional programs mediated by a key developmental regulator - Brachyury. Embryonic stem cells were differentiated into Brachyury-positive mesoendoderm cells. And, ChIP-seq experiments were carried out by two independent Brachyury antibodies.

2014-03-10 | E-GEOD-54978 | biostudies-arrayexpress

In vivo T-box Transcription Factor Profiling Reveals Joint Regulation of Embryonic Neuro-mesodermal Bipotency.

Project description:We defined genome-wide regulatory inputs of the T-box transcription factors Brachyury (Xbra), Eomesodermin (Eomes) and VegT that maintain neuro-mesodermal stem cells and determine their bipotential fates in the Xenopus tropicalis frog embryo. Binding profiles for Xbra, Eomes and VegT in X. tropicalis embryos (ChIP-Seq)

2013-09-19 | E-GEOD-48560 | biostudies-arrayexpress

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