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Expression data from umbilical cord blood and peripheral blood stem cell-derived monocytes of human

ABSTRACT: Expression data from umbilical cord blood and peripheral blood stem cell-derived monocytes of human

PROVIDER: PRJNA528106 | ENA |

REPOSITORIES: ENA

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Expression data from umbilical cord blood and peripheral blood stem cell-derived monocytes of human

Project description:To identify the differences between human umbilical cord blood and peripheral blood monocytes, we performed unsupervised bioinformatic analyses by microarrays.

2021-05-18 | GSE128562 | GEO

Comparative gene expression analyses of purified CD14+ monocytes from human umbilical cord blood, CD14+ monocytes from adult peripheral blood and the cell therapy product DUOC-01

Project description:DUOC-01 is a macrophage-like cell therapy product manufactured from human umbilical cord blood (CB) mononuclear cells (MNCs) and intended for use in treatment of demyelinating diseases. During the 21-day manufacturing process, most initiating MNCs die in culture, but a highly active, adherent population of cells that resemble hematopoietic lineage macrophage in morphology, phagocytic activity, and antigen expression emerges; this population comprises the final DUOC-01 product [Kurtzberg et al. 2015]. Using the cuprizone-mediated murine demyelination model, we have found that DUOC-01 cells are capable of accelerating the remyelination process. In the same demyelination model, human cord blood-derived CD14+ cells (CBCD14) were less active. In another cellular therapy project, we have found that CBCD14 protects brain cells from oxygen-glucose-deprivation (OGD), but adult peripheral blood-derived CD14+ monocytes (PBCD14) did not. To address the functional differences between these cell types, we have analyzed differential gene expression by these three cell types. We used four cord blood (CB) samples (from Carolinas Cord Blood Bank) that were collected within 24hrs of delivery. Then, highly purified CB CD14+ monocytes were isolated by flow sorting. We collected the peripheral blood (PB) from four healthy volunteers and isolated the purified PB CD14+ monocytes by cell sorting. Three separate batches of cultured DUOC-01 cells were collected from Robertson Cell and Translational Cell Therapy cGMP facility. Total RNAs were isolated at the same time and kept frozen. RNA samples were given to Duke Microarray core facility to analyse the gene expression profile using Affymetrix GeneChipÂ® Human Genome U133 Plus 2.0 Assay.

2016-01-13 | E-GEOD-76803 | biostudies-arrayexpress

Human Regulatory T cells from Umbilical Cord Blood Display Increased Repertoire Diversity and Lineage Stability Relative to Adult Peripheral Blood

Project description:Single-cell RNA and TCR sequencing data from sorted Tregs in umbilical cord blood and adult peripheral blood, both pre- and post-expansion.

2020-03-31 | GSE147794 | GEO

Comparative gene expression analyses of purified CD14+ monocytes from human umbilical cord blood, CD14+ monocytes from adult peripheral blood and the cell therapy product DUOC-01

2016-01-13 | GSE76803 | GEO

Transcriptomic profiling of 27-year cryopreserved umbilical cord blood HSCs/HPCs

Project description:Umbilical cord blood banking is critical for the success of umbilical cord blood transplants. Here we analyzed transcriptomic differences between 27-year cryopreserved umbilical cord blood hematopoietic stem cells (HSCs) and multipotent progenitor cells (MPPs) and those derived from fresh cord blood. We also leveraged differences in engraftment capacity to examine the transcriptomes of HSCs/HPCs defined by engraftment capacity, demonstrating the feasibility of this approach for identifying potency markers to aid in the selection of cord blood units for transplantation and revealing novel potential regulators of cord blood HSC/HPC engraftment.

2023-10-04 | GSE225699 | GEO

Transcriptional profile of human neonatal cord blood and adult peripheral blood dendritic cells

Project description:A phenotypically and functinoally distinct subset of human blood dendritic cells expressing CD11b is specific of the neonatal environment. We have employed whole genome microarray expression profiling to identify the specific gene signature of CD11b+ cord blood dendritic cells as compared to their adult peripheral blood counterparts. Peripheral blood adult cDC2 (CD20- CD11c+ CD14- BDCA1+ CD11b- ), neonatal cord blood cDC2 (CD20- CD11c+ CD14- BDCA1+ CD11b-) and neonatal cord blood cDC2b (CD20- CD11c+ CD14- BDCA1+ CD11b+) were FACS purified from BDCA1+ magnetically. Neonatal monocytes (CD11c+ CD14+) and neonatal naive T cells (CD3+ CD4+ CD56- CD25- CD45RO-) were used as controls.

2018-09-21 | GSE104110 | GEO

Human umbilical cord blood-derived mesenchymal stem cells from 2 donors

Project description:screening of signature deterimes the individual variations in the therapeutic efficacy of human umbilical cord blood-derived mesenchymal stem cells There is paucity of information whether human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) from separate donors might have different effects on improving myocardial repair after myocardial infarction (MI).

2011-02-19 | GSE27414 | GEO

Transcription profiling of human peripheral blood monocytes after constitutive adhesion to naive human umbilical vein endothelial cells (HUVEC) to investigate changes in gene expression patterns

Project description:Investigation of the alterations in the gene expression pattern of peripheral blood monocytes after constitutive adhesion to naive human umbilical vein endothelial cells

2007-03-10 | E-MEXP-854 | biostudies-arrayexpress

Umbilical cord blood artificial mixtures validation dataset: An Enhanced DNA Methylation Library for Deconvoluting Peripheral Blood

Project description:Umbilical cord blood artificial mixtures validation dataset: An Enhanced DNA Methylation Library for Deconvoluting Peripheral Blood

| PRJNA750131 | ENA

Expression data from Cord blood and Peripheral blood derived macrophages

Project description:The risk of sepsis is particularly high in neonates compared to older children and adults; however the reasons behind are incompletely understood. Macrophages are at the front line of the innate immune system and differentiate from monocytes after infiltrating the infected tissue. We studied transcriptional differences between cord blood-derived in vitro activated macrophages and adult peripheral blood derived in vitro actiavted macrophages. We used microarrays to detail the global programme of gene expression in cord blood derived M(IL-10) and M(IFN-γ) and in adult blood derived M(IL-10) and M(IFN-γ)macropahges

2018-12-19 | GSE124105 | GEO

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