Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression comparison of resting human peripheral-blood NK cells and activated counterparts

ABSTRACT: Gene expression comparison of resting human peripheral-blood NK cells and activated counterparts

PROVIDER: PRJNA528508 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression comparison of resting human peripheral-blood NK cells and activated counterparts

Project description:Resting NK cells are notoriously difficult to transduce, especially using the VSVG lentivirus pseudotype. When NK cells are expanded and activated using the NKAES system, there is a modest improvement of transduction rates compared to their resting NK counterparts. Still, the transduction efficiency remains poor for robust development of NK cell-based cancer immunotherapy. We envisaged to have a genome-wide expression profile of resting and activated NK (NKAES) cells in order to improve currrent transduction protocols .

2019-11-28 | GSE128696 | GEO

Resting and activating NK cells_gene signatures

Project description:Genome-wide transcriptional profiling was used to identify gene expression signatures and pathways in resting and IL2 activated NK cell isolated from peripheral blood of healthy donors Keywords: Time series_NK cells gene profile

2007-08-01 | GSE8170 | GEO

A comparison of the transcriptional profiles of resting NK cells and ex vivo expanded NK cells

Project description:NK cells are cytotoxic lymphocytes that play an important role in the innate immune response. The immune response of NK cells was shown under activated conditions. In order to identify such patterns, we performed RNA sequencing to confirm the gene expression profiles in NK cells expanded ex vivo compared to that of resting NK cells.

2023-05-17 | GSE222740 | GEO

mRNA expression profiles of resting and IL-15 activated murine NK cells

2007-05-15 | GSE7764 | GEO

Effect of resting or activated human regulatory T cells (Tregs) on human natural killer (NK) cell differentiation in vitro.

Project description:Activated but not resting Tregs affect NK cell differentiation. We used microarray analysis to study differences of gene expression of NK cell differentiation in the presence of resting or activated Tregs.

2016-06-08 | GSE55091 | GEO

Differential Expressions of miRNAs in resting and activated human in vitro-differentiated NK cells

Project description:To investigate miRNA expressions of miRNA upon activation. In vitro-differentiated human NK cells were freshly incubated in the presence of IL15 after 24h deprivation of IL-15. The cells were harvested at the times (0h, resting-Sample 1 and 2; 6h, activated-Sample 3 and 4) and analyzed by microarray.

2011-11-21 | E-GEOD-32411 | biostudies-arrayexpress

Microarray analysis of resting Nkrp1g+ NK cells versus resting Nkrp1g- NK cells from spleens of female BALB/c mice.

Project description:Natural Killer Gene Complex (NKC)-encoded immunomodulatory C-type lectin-like receptors (CTLR) include members of the NKRP1 and CLEC2 gene families, which constitute genetically linked receptor-ligand pairs and are thought to allow for NK cell-mediated immunosurveillance of stressed or infected tissues. The mouse CTLR Nkrp1g was previously shown to form several receptor-ligands pairs with the CLEC2 proteins Clr-d, Clr-f, and Clr-g, respectively. Recently, we demonstrated a gut-restricted expression of Clr-f on intestinal epithelial cells that is spatially matched by Nkrp1g on subsets of intraepithelial lymphocytes (Leibelt et al., 2015). We now investigated expression and ligand interaction of Nkrp1g in the splenic compartment, and found an exclusive expression of Nkrp1g on a small subset of NK cells that upregulates Nkrp1g after cytokine exposure. To further characterize NKrp1g+ NK cells, we performed microarray analysis of resting Nkrp1g+ versus Nkrp1g- splenic NK cells that were purified by FACsorting. Total RNA isolation was followed by an Affymetrix microarray analysis.

2017-11-21 | E-MTAB-6223 | biostudies-arrayexpress

Transcription profiling of mouse resting and IL-15 activated natural killer cells

Project description:Murine NK cells were compared at rest and following 24 hours of IL-15 stimulation for their mRNA expression profiles on the Affymetrix MOE430_2 microarray platform. Additional comparators included resting bulk splenocytes. Experiment Overall Design: Three pairs of flow sorted murine NK cells (C57Bl/6) were analzyed at rest or after 24 hours of IL-15 stimulation. Three biological replicates of each condition (resting or IL-15) are included in this anlaysis. In addition, bulk resting splenocytes were used (2 biological replicates with 2 chip replicates for a total of 4 replicates).

2009-04-28 | E-GEOD-7764 | biostudies-arrayexpress

Human resting natural killer (NK) cells, activated NK cells and their secreted small EVs have a specific miRNA repertoire with a distinct pattern of non-templated post-transcriptional modifications

Project description:NGS-based assesement of miRNA expression and post-transcriptional modification kinetics in human primary resting and activated natural killer (NK) cells and their released small EVs

2022-08-17 | GSE185171 | GEO

resting and IL2 activated human natural killer cells

Project description:The aim of the study was to investigate the activation of human NK cells by IL2 through analyzing the global gene expression at different time points (0, 2, 8 and 24 hours) after culture with the cytokine IL2 at 100 IU/ml. NK cells with the CD56+/CD16+ and CD3- phenotype were negatively selected by immunomagnetic beads and re-examined by flow-cytometry to ensure greater than 90% purity . Keywords: resting and IL2 activated NK cells(time series)

2007-08-07 | GSE8059 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data