Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genome-scale DNA methylation analysis for blood-based colorectal cancer detection

ABSTRACT: Genome-scale DNA methylation analysis for blood-based colorectal cancer detection

PROVIDER: PRJNA529690 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Genome-scale DNA methylation analysis for blood-based colorectal cancer detection

Project description:Aberrant DNA hypermethylation of CpG islands occurs frequently throughout the genome in human colorectal cancer (CRC). A genome-scale DNA hypermethylation analysis technique using plasma is attractive for the noninvasive early detection of CRC. The methylated CpG tandems amplification and sequencing (MCTA-Seq) method was applied to plasma samples from 147 CRC patients and 134 controls in which 5 from the CRC patients and 2 from the control subjects failed to pass a quality control value and were excluded from further analysis. The capacity of the method for discriminating CRC and hepatocellular carcinoma (HCC) was assessed. We identified many DNA methylation markers including known (e.g., SEPT9 and IKZF1) and novel (e.g., EMBP1, KCNQ5 and CHST11) CpG islands for detecting CRC in blood. A panel of 80 markers significantly discriminated early stage CRC and controls with a sensitivity of 78% and a specificity of 90%. This method can efficiently distinguish between early stage CRC and HCC. MCTA-Seq is a promising method for the noninvasive detection of CRC that also shows great potential for identifying the tissue of origin of cancer.

2019-05-31 | GSE124600 | GEO

Blood based expression of colorectal cancer and healthy controls

Project description:The immune system is crucial in regulating colorectal cancer tumorigenesis. Identification of immune-related transcriptomic signatures derived from the peripheral blood of colorectal cancer patients will provide insights into colorectal cancer pathogenesis and suggest novel clues to potential colorectal cancer immunotherapy strategies. We used microarrays to detail the blood-based gene expression of colorectal cancer patients and healthy controls to identify the colorectal cancer-specific immune genes potentially diagnostic for colorectal cancer.

2021-01-05 | GSE164191 | GEO

Blood Gene Expression Profiling in Colorectal Cancer

Project description:In this work, we performed gene expression profiling for twenty-paired blood samples collected from healthy controls before and after colonoscopy, and twenty blood samples collected from<br>colorectal cancer patients after colonoscopy. The aim of this study is to determine how significant the colonoscopy procedure may impact the global gene expression in human peripheral blood, and whether this colonoscopy induced variability would bias the biomarker research for colorectal cancer early detection.

2013-10-01 | E-MEXP-3756 | biostudies-arrayexpress

Genome-scale analysis of aberrant DNA methylation in colorectal cancer

Project description:To characterize DNA methylation-based subgroups in colorectal cancer, we performed genome-scale DNA methylation profiling of 125 colorectal tumor samples and 29 histologically normal-adjacent colonic tissue samples using the Illumina Infinium DNA methylation assay, which assesses the DNA methylation status of 27,578 CpG sites located at the promoter regions of 14,495 protein-coding genes. We identified four DNA methylation-based subgroups of CRC using model-based cluster analyses. Each subtype shows characteristic genetic and clinical features, indicating that they represent biologically distinct subgroups. Bisulfite converted DNA from fresh frozen 125 colorectal tumors and 29 adjacent normal tissues were hybridized to the Illumina Infinium 27k Human Methylation Beadchip v1.2

2011-06-03 | E-GEOD-25062 | biostudies-arrayexpress

Genome-scale discovery of DNA-methylation biomarkers for blood-based detection of colorectal cancer.

Project description: Not available

| S-EPMC3508917 | biostudies-literature

Genome-scale analysis of aberrant DNA methylation in colorectal cancer

2011-06-03 | GSE25062 | GEO

Tissue and blood microRNA array profiles of colorectal cancer patients

Project description:microRNAs (miRNAs) are short, non-coding RNA molecules that act as regulators of gene expression. Circulating blood miRNAs offer great potential as cancer biomarkers. The objective of the study was to correlate the differential expression of miRNAs in tissue and blood in the identification of biomarkers for early detection of colorectal cancer (CRC).

2013-12-30 | GSE39845 | GEO

Tissue and blood microRNA array profiles of colorectal cancer patients

2013-12-30 | E-GEOD-39845 | biostudies-arrayexpress

Blood based expression of colorectal cancer and healthy controls

Project description:Blood based expression of colorectal cancer and healthy controls

| PRJNA689481 | ENA

Blood-Based Detection of Colorectal Cancer Using Cancer-Specific DNA Methylation Markers.

Project description: Not available

| S-EPMC7823774 | biostudies-literature