Project description:This experiment analyzes the changes in expression of twelve days old Arabidopsis roots after 10 hours of beet cyst nematode Heterodera schachtii treatment
Project description:In order to polish a long-read genome assembly, short-read illumina data was obtained from Heterodera schachtii cysts (Woensdrecht population from IRS, the Netherlands). Cysts where obtained from infected plant material. Nematodes were cleaned using a sucrose gradient centrifugation step. Thereafter DNA was extracted and used for library preparation and sequencing by Illumina NextSeq500.
Project description:We compared the gene expression of wild-type Col-0, tcp9, tcp20, and the double mutant tcp9tcp20. We either infected or mock-infected the plants with the beet cyst nematode Heterodera schachtii and measured the root transcriptome after 24, 48, and 72 hours post infection using RNA-seq. The aim of the experiment was to determine whether the tcp mutatants affected gene expression patterns induced by nematode infection.
