Project description:Limited understanding of the immunopathogenesis of human herpesvirus 6B (HHV-6B) has prevented its acceptance as a pulmonary pathogen after hematopoietic cell transplantation (HCT). We conducted a prospective multicenter study of patients undergoing bronchoalveolar lavage (BAL) for pneumonia after allogeneic HCT. We tested blood and BAL fluid (BALF) for HHV-6B DNA and mRNA transcripts associated with lytic infection and performed RNA-seq on paired blood. Among 116 participants, HHV-6B DNA was detected in 37% of BALs, 49% of which had HHV-6B mRNA detection. We established an HHV-6B DNA threshold (≥2.3 log10copies/ml in BALF) that was highly predictive of HHV-6B mRNA detection and increased risk for death from respiratory failure (adjusted HR, 2.35; 95% CI, 1.08-5.11). Participants with HHV-6B DNA in BALF exhibited distinct host gene expression signatures, notable for enriched interferon signaling pathways in participants clinically diagnosed with idiopathic pneumonia. These data implicate HHV-6B as a pulmonary pathogen after allogeneic HCT.

2023-12-18 | GSE244210 | GEO

Search for apoptosis-related transcriptome markers associated with herpesvirus infections in peripheral blood leukocytes

Project description:Search for apoptosis-related transcriptome markers associated with herpesvirus infections in peripheral blood leukocytes

| PRJNA432422 | ENA

RNA sequencing analysis of HHV-6A-infected T cells

Project description:To determine if HHV-6A infection affects cell metabolism of host cells, we conducted a global RNA sequencing analysis in HHV-6A infected cells

2020-05-05 | GSE149808 | GEO

Leukocytes

Project description:Leukocytes

| PRJNA576499 | ENA