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MRNA sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

ABSTRACT: mRNA sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

PROVIDER: PRJNA530985 | ENA |

REPOSITORIES: ENA

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Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

Project description:Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

| PRJNA487079 | ENA

mRNA sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

Project description:Col-0 plants were transformed with MSH1 RNAi construct. Transgene positive plant was self pollinated and transgene was segregated in subsequent generation, and screened for presence of transgene using PCR assay. Of transgene null plants, 20% plants displayed delayed in flowering, smaller in size, and lighter green termed as memory phenotype. Memory plants were self pollinated for six generations and plants from generation 1 and 5 were sequenced for RNASeq.

2020-04-03 | GSE129343 | GEO

Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis

Project description:Col-0 plants were transformed with MSH1 RNAi construct. Transgene positive plant was self pollinated and transgene was segregated in subsequent generation, and screened for presence of transgene using PCR assay. Of transgene null plants, 20% plants displayed delayed in flowering, smaller in size, and lighter green termed as memory phenotype. While rest transgene null plants displayed normal phenotype termed as non-memory.

2019-08-06 | GSE118874 | GEO

Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis [generation 2 to 6]

Project description:Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis [generation 2 to 6]

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mRNA sequencing of msh1 memory line in Arabidopsis

Project description:Germination of the memory line and isogenic Col-0 wild type on media containing 100 uM 5-azacytidine alleviated the phenotype differences between the two lines, resulting in similar growth rates, here we generate gene expression profile for wild type and msh1 memory line under 100 uM 5-azacytidine treatment to investigate the effect of 5-azacytidine on msh1 memory gene expression profile

2019-01-12 | GSE109164 | GEO

Bisulfite (methylome) sequencing of msh1 memory line and non-memory (normal looking) sibling plants with isogenic Col-0 wild-type control in Arabidopsis [generation 2 to 6]

2020-04-03 | GSE129303 | GEO

Bisulfite (methylome) sequencing of msh1 memory line treated with 5-azacytidine in Arabidopsis

Project description:Germination of the memory line and isogenic Col-0 wild type on media containing 100 uM 5-azacytidine alleviated the phenotype differences between the two lines, resulting in similar growth rates. Here we generate methylome profile for wild type and msh1 memory line under 100 uM 5-azacytidine treatment to investigate the effect of 5-azacytidine on msh1 memory line methylation profile.

2019-05-18 | GSE114665 | GEO

Bisulfite methylome sequencing of msh1 memory line in tomato

Project description:MSH1 depletion caused whole genome wide Methylome changes in Arabidopsis, to conduct comparative genomic analysis, here we generate methylome for MSH1-RNAi line and transgene segregated out msh1 memory line in tomato

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MSH1 disruption in plants triggers RdDM-mediated reprogramming of phenotypic plasticity (Bisulfite-Seq II)

Project description:To generate msh1 memory revertant population, msh1 memory plant was used to pollinate Col-0 to generate F1 population. Derived F1 progenies were self-pollinated to generate F2 populations. F2 plants were self-pollinated to produce F3 families that were segregationg for the msh1 memory phenotype (small dwarf plants with delayed flowering).

2022-07-08 | GSE192432 | GEO

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