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Single cell sequencing of de-differentiated dermal adipocytes

ABSTRACT: Single cell sequencing of de-differentiated dermal adipocytes

PROVIDER: PRJNA531057 | ENA |

REPOSITORIES: ENA

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Single cell sequencing of de-differentiated dermal adipocytes

Project description:Purpose: The goals of this study are to compare the de-differentiated dermal adipocytes with normal skin fibroblasts. Methods: Library prepared followed by 10X Genomics standard protocol. Transcriptome was generated by high throughput sequencing.

2019-09-16 | GSE129371 | GEO

Single-cell RNA-seq of de-differentiated mammary adipocytes during lactation

Project description:Adipose tissue in the mammary gland undergoes dramatic remodeling during reproduction. Adipocytes are replaced by mammary alveolar structures during pregnancy and lactation, then reappear upon weaning. Here, we reveal that adipocytes in the mammary gland de-differentiate into Pdgfrα+ preadipocyte- and fibroblast-like cells during pregnancy, and remain de-differentiated during lactation. Upon weaning, de-differentiated fibroblasts proliferate and re-differentiate into adipocytes. In order to determine the molecular signature of these de-differentiated adipocytes in the mammary gland, we compared these cells with classical adipocytes. Using the AdipoChaser-mT/mG system, we pre-labeled mature adipocytes with GFP expression to characterize the features of these de-differentiated adipocytes (Figure 4A), and then purified CD31-/CD45-/PDGFRα+/Tomato+ and CD31-/CD45-/PDGFRα+/GFP+ cells from the stromal vascular fraction (SVF) of lactating mammary gland at the peak of lactation through FACS. Gene expression analyses showed that the CD31-/CD45-/PDGFRα+/Tomato+ cells were indeed enriched with Tomato expression, while the CD31-/CD45-/PDGFRα+/GFP+ cells were enriched with GFP expression (Figure 4C). We then collected CD31-/CD45-/PDGFRα+/GFP+ cells as single cells for subsequent single cell RNA-sequencing analysis (Figure 4D-G, Supplemental. Figure S1A-G). After the flow sorting and single cell RNA amplification, 26 CD31-/CD45-/PDGFRα+/GFP+ cells passed the quality control, and these cells were used for single-cell RNA-sequencing analysis. Due to technical difficulties in sorting single mature white adipocyte through flow cytometry, adipocytes differentiated from the immortalized murine-derived brown pre-adipocyte cell line were used as mature adipocyte control (Pradhan et al., 2017). Additionally, we also included population RNA-seq experiments, i.e. three mature white adipocyte samples, two GFP+, and six GFP- ones.

2018-06-17 | E-MTAB-6818 | biostudies-arrayexpress

Single-cell RNA-seq of de-differentiated mammary adipocytes during lactation

Project description:Single-cell RNA-seq of de-differentiated mammary adipocytes during lactation

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Transcriptome sequencing of purified dermal adipocytes and inguinal adipocytes

Project description:Purpose: The goals of this study are to compare the transcription difference between purified dermal adipocytes and inguinal adipocytes Methods: Transcriptome of purified dermal adipocytes and inguinal adipocytes were generated by deep sequencing, using Illumina Hiseq 2500 v3 sequencing system.

2019-09-16 | GSE128719 | GEO

Single cell sequencing of de-differentiated adipocytes induced by breast tumor

Project description:Purpose: The goals of this study are to determine adipocyte de-differentiation and the fate of these de-differentiated cells in breast tumor. Methods: Library prepared followed by 10X Genomics standard protocol. Transcriptome was generated by high throughput sequencing.

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Transcriptome sequencing of purified dermal adipocytes and inguinal adipocytes

Project description:Transcriptome sequencing of purified dermal adipocytes and inguinal adipocytes

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Project description:Purpose: The goals of this study are to compare the transcription changes of dermal adipocytes during hair cycling. Methods: Transcriptome of purified dermal adipocytes were generated by deep sequencing, using Illumina Novaseq 6000 sequencing system.

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Small RNA profiling of differentiated and de-differentiated vascular smooth muscle cell

Project description:We report the application of small RNA sequencing for high-throughput profiling of small RNA under 75 bp in vascular smooth muscle cell. By a reading depth of 30M and single stranded sequencing, we generated the small RNA signature on differentiated and de-differentiated vascular smooth muscle cell induced by PDGF-BB and H3K4me2 editing. We found that PDGF-BB and H3K4me2 editing induced de-differentiation modulated miRNA profile significantly, which was demonstrated at least in part responsible for modulated vascular smooth muscle cell phenotype.

2022-04-01 | GSE186972 | GEO

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2018-02-06 | E-MTAB-6469 | biostudies-arrayexpress

Expression data from differentiated 3T3-L1 pre-adipocytes.

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