Project description:The increased fitness when grown with pyruvate as carbon source in response to lanthanum supplementation was used as starting point to study genes induced in Beijerinckiaceae bacterium RH AL1 upon exposure to lanthanum.
Project description:Beijerinckiaceae bacterium RH AL1 was grown exponentially in with methanol (1% [v/v]) as carbon source and lanthanum (1µM) as necessary growth supplement. Harvested biomass was subjected to RNA extraction, mRNA-enrichment and Illumina sequencing library preparation for subsequent RNA-Seq analysis. The scope of this gene expression analysis was to validate the expression of genes linked to pathways that are involved in C1-metabolism.
Project description:Transcriptome analysis of Beijerinckiaceae bacterium RH AL1 grown with pyruvate as the carbon source in response to lanthanum supplementation
