Project description:Carbendazim (Methyl benzimidazol-2-ylcarbamate; MBC) is an antimitotic drug used for broad-spectrum fungicide, antineoplastic and mutagen in microbial breeding. Using a customized SNP microarray technology, this work revealed the effect of MBC on genomic instability (loss of heterozygosity, chromosomal rearrangements and aneuploidy) in the diploid yeast Saccharomyces cerevisiae JSC25.
Project description:Pichia caribbica, which was isolated by our laboratory, was cultured at 4 °C on nutrient yeast dextrose agar (NYDA).A loop of the culture was inoculated in nutrient yeast dextrose broth and incubated on a rotary shaker at 180 rpm at 28 °C for 20 h.