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Establishment of bovine expanded potential stem cells

ABSTRACT: Establishment of bovine expanded potential stem cells

PROVIDER: PRJNA532647 | ENA |

REPOSITORIES: ENA

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Establishment of bovine expanded potential stem cells

Project description:Embryonic stem cells (ESCs) and induced pluripotent stem cells have the potential to differentiate to all cell types of an adult individual and are useful for studying development and for translational research. However, extrapolation of mouse and human ESC knowledge to deriving stable ESC lines of domestic ungulates and large livestock species has been challenging. In contrast to ESCs that are usually established from the blastocyst, mouse expanded potential stem cells (EPSCs) are derived from four-cell and eightcell embryos. We have recently used the EPSC approach and established stem cells from porcine and human preimplantation embryos. EPSCs are molecularly similar across species and have broader developmental potential to generate embryonic and extraembryonic cell lineages. We further explore the EPSC technology for mammalian species refractory to the standard ESC approaches and report here the successful establishment of bovine EPSCs (bEPSCs) from preimplantation embryos of both wild-type and somatic cell nuclear transfer. bEPSCs express high levels of pluripotency genes, propagate robustly in feeder-free culture, and are genetically stable in long-term culture. bEPSCs have enriched transcriptomic features of early preimplantation embryos and differentiate in vitro to cells of the three somatic germ layers and, in chimeras, contribute to both the embryonic (fetal) and extraembryonic cell lineages. Importantly, precise gene editing is efficiently achieved in bEPSCs, and genetically modified bEPSCs can be used as donors in somatic cell nuclear transfer. bEPSCs therefore hold the potential to substantially advance biotechnology and agriculture.

2021-04-12 | GSE129760 | GEO

Establishment in Culture of Mouse Expanded Potential Stem Cells

Project description:Establishment in Culture of Mouse Expanded Potential Stem Cells

| PRJEB6168 | ENA

Establishment of Porcine and Human Expanded Potential Stem Cells (ChIP-seq)

Project description:Establishment of Porcine and Human Expanded Potential Stem Cells (ChIP-seq)

| PRJEB31243 | ENA

Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)

Project description:Establishment of Porcine and Human Expanded Potential Stem Cells (bulk RNA-seq)

| PRJEB31242 | ENA

Establishment of Porcine and Human Expanded Potential Stem Cells (Single-Cell RNA-seq)

Project description:Establishment of Porcine and Human Expanded Potential Stem Cells (Single-Cell RNA-seq)

| PRJEB31259 | ENA

Establishment of Bovine Induced Pluripotent Stem Cells

Project description:Establishment of Bovine Induced Pluripotent Stem Cells

| PRJNA750110 | ENA

Establishment in Culture of Mouse Expanded Potential Stem Cells

Project description:The zygote and blastomeres of a cleavage stage mouse embryo have the capacity to differentiate to all lineages in the embryo proper and extraembryonic tissues and are considered totipotent. Mouse ESCs can differentiate to embryonic lineages but have restricted potential to trophoblasts. We report here that cultures of a new type of stem cells with expanded potential (EPSCs) can be established from in vitro cultured mouse preimplantation embryos and individual blastomeres, from directly converting mouse ESCs, or from genetically reprogramming somatic cells. EPSCs differentiate to trophoblasts in vitro, and a single EPSC contributes in chimeras to both extraembryonic lineages including the placenta trophoblasts and the embryo proper. Molecular analyses reveal that EPSCs express core pluripotency factors similar to ESCs, but have enriched signature of preimplantation blastomeres. The data described here suggest that similar cell lines from other mammalian species might also be established in culture and stably maintained.

2014-08-27 | E-ERAD-285 | biostudies-arrayexpress

Establishment of bovine expanded potential stem cells.

Project description: Not available

| S-EPMC8053967 | biostudies-literature

Establishment of Porcine and Human Expanded Potential Stem Cells (ChIP-seq)

Project description:Despite intensive efforts, establishing porcine embryonic stem cells have been challenging. We recently derived mouse expanded potential stem cells (EPSCs) from individual blastomeres by inhibiting the activity of critical molecular pathways that predisposes lineage differentiation in the mouse preimplantation embryo. EPSCs had enriched molecular signatures of blastomeres and possessed the developmental potency to all embryonic and extraembryonic cell lineages. In this study, we report the derivation of porcine EPSC (pEPSC) lines either directly from preimplantation embryos or by reprogramming fetal fibroblasts. Under similar culture conditions, human ESCs and iPSCs can be converted, or somatic cells are directly reprogrammed, to EPSCs (hEPSCs) that display the molecular and functional attributes reminiscent of pEPSCs. Here, we performed ChIP-seq experiments of H3K4me3 and H3K27me3 to characterise the epigenetic signatures of the EPSCs.

2019-04-01 | E-MTAB-7252 | biostudies-arrayexpress

Establishment of bovine trophoblast stem cells [WGBS]

Project description:Here we report that a chemical cocktail (LCDM: hLIF, CHIR99021, DiM and MiH) previously reported for extended potential pluripotent stem cells enables the de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs exhibit transcriptomic and epigenetic features characteristic of trophectoderm cells from bovine embryos and retain developmental potency to differentiate into functional trophoblasts in vitro and in vivo

2023-07-20 | GSE220924 | GEO

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