Project description:To identify pathways regulating NSC quiescence, with a possible link to quiescence depth, we used double transgenic Tg(her4:drfp);Tg(mcm5:gfp) fish and paradigms predicted to highlight deep vs shallower quiescence. In adult fish (3 month-post-fertilization -mpf-), we performed bulk RNA sequencing on FACS sorted RFPhigh,GFPneg qNSCs (expressing strongly her4, signing NSCs transcriptionally remote from activation), and activated NSCs.
Project description:Primitive neural stem cells (NSCs) could be derived from pluripotent mouse embryonic stem (ES) cells, and then differentiate into definitive-type neural stem cells which resemble NSCs obtained from the central nervous system. Hence, primitive NSCs define an early stage of neural induction and provide a model to understand the mechanism that controls initial neural commitment. In this study, we performed microarray assay to analyze the global transcriptional profiles in mouse ES cell-derived primitive and definitive NSCs and to depict the molecular changes during the multi-staged neural differentiation process. Primitive NSCs derived directly from ESCs in Lif (p-NSC_L), primitive NSCs that were sub-cultured in the presence of Lif and FGF (p-NSC_LF), as well as definitive NSCs derived from primitive NSCs in medium containing FGF and EGF, were collected for RNA extraction and hybridization on Affymetrix microarrays. Mouse ESCs and NSCs obtained from mouse embryonic brain (E11.5) were included for controls. For each cell type, we collected two biological replicate samples for microarray analysis.
Project description:Primitive neural stem cells (NSCs) could be derived from pluripotent mouse embryonic stem (ES) cells, and then differentiate into definitive-type neural stem cells which resemble NSCs obtained from the central nervous system. Hence, primitive NSCs define an early stage of neural induction and provide a model to understand the mechanism that controls initial neural commitment. In this study, we performed microarray assay to analyze the global transcriptional profiles in mouse ES cell-derived primitive and definitive NSCs and to depict the molecular changes during the multi-staged neural differentiation process.
Project description:Rat NSCs were isolated from developing whole brian of the SD rat embryos at day 13.5. In the NSC/GFP-EMSC co-culture system, the NSCs showed significantly enhanced neuronal differentiation rather than astrocytic commitment, in comparison with the mono-cultured NSCs. In order to reveal the gene expression profiles of NSCs under these two diffferent culture conditions, we used microassrays to examine the global programme of gene expression of NSCs under these two diffferent culture conditions.
Project description:We generated a dataset in which we compared the bulk transcriptome of qNSCs between 1.5 and 3.5 mpf fish, stages between which deeper quiescence is progressively instated in order to find differences between deeply and shallow quiescent NSC
Project description:This SuperSeries is composed of the following subset Series: GSE8542: BY4742 Quiescent and Non-quiescent replicates GSE8558: Proteinase K treatment of Q and NQ cells GSE8559: S288c Quiescent and Non-quiescent replicates GSE8560: Mutant1 GSE8561: Mutant2 Refer to individual Series
Project description:Quiescent neural stem cells (NSCs) in the adult ventricular-subventricular zone (V-SVZ) undergo activation and divide to generate neurons and glia. Here we show that Platelet-derived Growth Factor Receptor beta (PDGFRβ) is expressed by quiescent and early activated adult V-SVZ NSCs, and maintains their quiescence. We further showed that selective deletion of PDGFRβ in adult V-SVZ NSCs leads to activation of quiescent NSCs. We performed RNA-seq on FACS-sorted V-SVZ quiescent, early activated and late activated NSCs, as well as cortical cells from adult 2-4 month old mice.